View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
IV, V, I, II, III
III, II, IV, V, I
III, IV, V, I, II
II, III, V, IV, I
I, II, IV, III, V
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Plasmids (or vectors) are important in biotechnology becausethey are
a vehicle for the insertion ofrecombinant DNA into bacteria.
surfaces for respiratoryprocesses in bacteria.
recognition sites onrecombinant DNA strands.
surfaces for protein synthesisin eukaryotic recombinants.
proviruses incorporated intothe host DNA
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Plasmids are put into bacterial cells by
restriction enzymes
DNA ligase
binding of cohesive stickyends
transformation
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Restriction enzymes usually
cut donor DNA evenly so smoothedges result
cut donor DNA but do notaffect plasmids
make staggered cuts atspecific sequences in DNA in both donor DNA and plasmid
are used in ligating plasmidsinto bacterial host cells
more than one of theabove
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After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
Restriction enzyme
DNA Ligase
Reverse transcriptase
DNA polymerase
Helicase
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It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
All organisms haveribosomes.
All organisms have the samegenetic code.
All organisms are made up ofcells.
All organisms have similarnuclei.
All organisms have transferRNA.
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Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
cut the plasmid withrestriction enzyme X and insert the fragments cut with Y into theplasmid.
cut the plasmid with enzyme Xand then insert the gene into the plasmid.
cut the DNA again withrestriction enzyme Y and insert these fragments into the plasmidcut with the same enzyme.
cut the plasmid twice withrestriction enzyme Y and ligate the two fragments onto the ends ofthe human DNA fragments cut with restriction enzyme X.
insert the fragments cut withX directly into the plasmid without cutting the plasmid.
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Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
They are used to make proteinsusing a cloned gene.
They contain a promotor.
They are the first plasmidtype used to clone a gene.
They contain aterminator.
More than one of the above isfalse.
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What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
If the eukaryotic genecontains introns the bacteria will not remove them and theresulting amino acid sequence will be different that that made by aeukaryote.
The bacteria may not fold theprotein correctly.
The bacteria may degrade theprotein.
All of the above are potentialproblems.
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Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
True
False
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Question 111 pts
Gene cloning is used to do all of the following except
Make insulin
Making genetically identicalanimals (e.g. Dolly the sheep)
Make vaccines
Perform Gene Therapy
Making genetically engineeredplants
Flag this Question
In your
View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
IV, V, I, II, III |
III, II, IV, V, I |
III, IV, V, I, II |
II, III, V, IV, I |
I, II, IV, III, V |
Flag this Question
Plasmids (or vectors) are important in biotechnology becausethey are
a vehicle for the insertion ofrecombinant DNA into bacteria. |
surfaces for respiratoryprocesses in bacteria. |
recognition sites onrecombinant DNA strands. |
surfaces for protein synthesisin eukaryotic recombinants. |
proviruses incorporated intothe host DNA |
Flag this Question
Plasmids are put into bacterial cells by
restriction enzymes |
DNA ligase |
binding of cohesive stickyends |
transformation |
Flag this Question
Restriction enzymes usually
cut donor DNA evenly so smoothedges result |
cut donor DNA but do notaffect plasmids |
make staggered cuts atspecific sequences in DNA in both donor DNA and plasmid |
are used in ligating plasmidsinto bacterial host cells |
more than one of theabove |
Flag this Question
After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
Restriction enzyme |
DNA Ligase |
Reverse transcriptase |
DNA polymerase |
Helicase |
Flag this Question
It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
All organisms haveribosomes. |
All organisms have the samegenetic code. |
All organisms are made up ofcells. |
All organisms have similarnuclei. |
All organisms have transferRNA. |
Flag this Question
Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
cut the plasmid withrestriction enzyme X and insert the fragments cut with Y into theplasmid. |
cut the plasmid with enzyme Xand then insert the gene into the plasmid. |
cut the DNA again withrestriction enzyme Y and insert these fragments into the plasmidcut with the same enzyme. |
cut the plasmid twice withrestriction enzyme Y and ligate the two fragments onto the ends ofthe human DNA fragments cut with restriction enzyme X. |
insert the fragments cut withX directly into the plasmid without cutting the plasmid. |
Flag this Question
Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
They are used to make proteinsusing a cloned gene. |
They contain a promotor. |
They are the first plasmidtype used to clone a gene. |
They contain aterminator. |
More than one of the above isfalse. |
Flag this Question
What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
If the eukaryotic genecontains introns the bacteria will not remove them and theresulting amino acid sequence will be different that that made by aeukaryote. |
The bacteria may not fold theprotein correctly. |
The bacteria may degrade theprotein. |
All of the above are potentialproblems. |
Flag this Question
Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
True |
False |
Flag this Question
Question 111 pts
Gene cloning is used to do all of the following except
Make insulin |
Making genetically identicalanimals (e.g. Dolly the sheep) |
Make vaccines |
Perform Gene Therapy |
Making genetically engineeredplants |
Flag this Question
In your