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What are the other components of the master mix besidesnucleotides and what are their functions?


Explain why the precise length target DNA sequence doesn't getamplified until the third cycle.

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Jarrod Robel
Jarrod RobelLv2
28 Sep 2019
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Related questions

What would happen if you mistakenly added some of the Instagene matrix to your PCR reaction tube?

Why is it necessary to have a primer on each side of the DNA segment to be amplified?

Describe the 3 main steps of each cycle of PCR amplification and what reactions occur at each temperature.

The first cycle does not yield the precise length target DNA sequence (the first cycle yields larger

fragments). Precise length double-stranded DNA is not formed until cycle 3. Why? (it may help to draw it out)

List all of the needed components of PCR and what the function is of each.

in a PCR amplification expleriment explain why the precise length target DNA sequence doesn

PCR

How does the quantity of DNA containing target region sequence change with each cycle?

In which cycle does the reaction first produce fragments of the length equal to the 2 primers + the distance between their annealing sites?

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