Compare DNA and RNA with regard to theirstructure, function, location, and activity. Ho do these moleculesdiffer with regard to the polymerases used to synthesize them?

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Reid WolffLv2

28 Sep 2019

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Related questions

The reason that there are Okazaki fragments during DNA replication is ultimately because:

A) Some parts of the chromosome are made of RNA and different polymerases have to be used

B) Polymerases can only synthesize in the 5' to 3' direction

C) There are only enough dNTP's to support quick replication on one strand, so the other strand lags behind it

D) It is necessary to "slow down" one of the strands so that replication does not occur before cells have a chance to grow

You have linear DNA fragments with T7 and T3 promoters in the antisense strand downstream of the PC2 open reading frame

This same piece of DNA also has SP6 and T3 promoters upstream of the PC2 open reading frame. Which enzyme would you use to transcribe an antisense riboprobe to assess PC2 expresssion?

A. T7 RNA polymerase

B. T3 RNA polymerase

C. SP6

D. T7 and T3 RNA polymerases

E. SP6 and T3 RNA polymerases

DNA polymerases are not able to prime replication, yet primase and other RNA polymerases can. Some geneticists have speculated that the inability of DNA polymerase to prime replication is due to its proofreading function. This hypothesis argues that proofreading is essential for the faithful transmission of genetic information and that, because DNA polymerases have evolved the ability to proofread, they cannot prime DNA synthesis. Explain why proofreading and priming functions in the same enzyme might be incompatible.