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9 Dec 2018

You have linear DNA fragments with T7 and T3 promoters in the antisense strand downstream of the PC2 open reading frame

This same piece of DNA also has SP6 and T3 promoters upstream of the PC2 open reading frame. Which enzyme would you use to transcribe an antisense riboprobe to assess PC2 expresssion?

A. T7 RNA polymerase

B. T3 RNA polymerase

C. SP6

D. T7 and T3 RNA polymerases

E. SP6 and T3 RNA polymerases

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Lelia LubowitzLv2

11 Dec 2018

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Related questions

Which type of RNA is unique to eukaryotes?

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QUESTION 2

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QUESTION 3

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QUESTION 4

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QUESTION 5

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QUESTION 6

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QUESTION 7

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QUESTION 9

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QUESTION 10

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please help i am a bit confused

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

Multiple answers to each question might be possible!

You decide to identify the CFTR mutation by analyzing the genomic DNA of your patients compared to healthy individuals. You specifically are looking to see whether a specific 3' gene truncation has occurred in the patients. You will determine this using hybridization techniques with samples from healthy and CF patients. Which of the following will allow you to accomplish this?

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