1. Name two similarities and two differences between thecellular processes of importing
protein into the ER and importing protein to the nucleus.
2. What proteins are directly involved in the transportation ofcargo in a clathrin-coated
vesicle?
a. Dynamin
b. Adaptins
c. Cargo receptors
d. t SNARES
3. Suppose a cell was treated with colchiine, a drug causingmicrotubule disassembly.
What would happen within the cell? Why?
Suppose there were a genetic defect in the genes that code forkinesins and dyneins.
What would happen within the cell? Why?
4. Which of the following are true about microtubules?
a. They are utilized by the disease causing bacteriaLysteria.
b. Instability in the minus-end of the microtubule is caused bythe hydrolization of GTP.
c. Centrosomes contain binding sites for microtubules composed atype of tubulin ring.
d. Cellular concentrations of tubulin are above the criticalconcentration for plus-end
polymerization.
e. Motor proteins can attach organelles to the microtubules.
5. Indicate which of the following statements are false:
a. Membrane fusion is energetically unfavorable and thusrequires ATP or similar high
energy molecule to occur
b. Clathrin is responsible for capturing specific molecules fortransport in coated vesicle
structures.
c. Receptor proteins that are incorporated into an endosome canbe retrieved back to the
membrane domain it originated from; can return to a new domainof the membrane; or
can enter the lysosome for digestion.
d. Transport vesicles occasionally bud from the trans Golginetwork to fuse with the
plasma membrane in a process called constitutive exocytosis.
e. Most components of the endocytic vesicle membrane eventuallyare returned to the
plasma membrane for reuse.
6. Match the following organelles with their function:
Organelle Bank:
a. Mitochondria
b. Endoplasmic reticulum
c. Nucleus
d. Lysosome
e. Endosome
f. Peroxisome
g. Golgi apparatus
_____ quality control of mRNA
_____ location of oxidative phosphorylation
_____ responsible for detoxifying organic molecules (livercells)
_____ houses and protects genetic material
_____ responsible for modification and sorting of proteins andlipids
_____ location of ATP synthesis
_____ responsible for sorting endocytosed materials
_____ site of degradation and digestion
_____ responsible for oxidizing toxic molecules
_____ location of lipid synthesis
_____ location of hormone synthesis (adrenal cells)
B. Which organelle(s) from the above list communicate(s) withother organelle(s)
through the use of vesicular transport? ________________
C. Which organelle(s) from the above list receive proteins madein the
cytosol?___________________
D. Through which organelle(s) must proteins pass to reach theorganelle(s) which do not
receive proteins made exclusively in thecytosol?______________
E. Entrance into which organelle(s) requires that proteinsunfold and snake through the
membrane? _________________
F. How do organelles maintain their position in the cytosol? Bespecific.
G. Give 2 advantages that eukaryotic cells gain by havingorganelles.
7. Which of the following statements are true regardingactin?
a. They are arranged in a
1. Name two similarities and two differences between thecellular processes of importing
protein into the ER and importing protein to the nucleus.
2. What proteins are directly involved in the transportation ofcargo in a clathrin-coated
vesicle?
a. Dynamin
b. Adaptins
c. Cargo receptors
d. t SNARES
3. Suppose a cell was treated with colchiine, a drug causingmicrotubule disassembly.
What would happen within the cell? Why?
Suppose there were a genetic defect in the genes that code forkinesins and dyneins.
What would happen within the cell? Why?
4. Which of the following are true about microtubules?
a. They are utilized by the disease causing bacteriaLysteria.
b. Instability in the minus-end of the microtubule is caused bythe hydrolization of GTP.
c. Centrosomes contain binding sites for microtubules composed atype of tubulin ring.
d. Cellular concentrations of tubulin are above the criticalconcentration for plus-end
polymerization.
e. Motor proteins can attach organelles to the microtubules.
5. Indicate which of the following statements are false:
a. Membrane fusion is energetically unfavorable and thusrequires ATP or similar high
energy molecule to occur
b. Clathrin is responsible for capturing specific molecules fortransport in coated vesicle
structures.
c. Receptor proteins that are incorporated into an endosome canbe retrieved back to the
membrane domain it originated from; can return to a new domainof the membrane; or
can enter the lysosome for digestion.
d. Transport vesicles occasionally bud from the trans Golginetwork to fuse with the
plasma membrane in a process called constitutive exocytosis.
e. Most components of the endocytic vesicle membrane eventuallyare returned to the
plasma membrane for reuse.
6. Match the following organelles with their function:
Organelle Bank:
a. Mitochondria
b. Endoplasmic reticulum
c. Nucleus
d. Lysosome
e. Endosome
f. Peroxisome
g. Golgi apparatus
_____ quality control of mRNA
_____ location of oxidative phosphorylation
_____ responsible for detoxifying organic molecules (livercells)
_____ houses and protects genetic material
_____ responsible for modification and sorting of proteins andlipids
_____ location of ATP synthesis
_____ responsible for sorting endocytosed materials
_____ site of degradation and digestion
_____ responsible for oxidizing toxic molecules
_____ location of lipid synthesis
_____ location of hormone synthesis (adrenal cells)
B. Which organelle(s) from the above list communicate(s) withother organelle(s)
through the use of vesicular transport? ________________
C. Which organelle(s) from the above list receive proteins madein the
cytosol?___________________
D. Through which organelle(s) must proteins pass to reach theorganelle(s) which do not
receive proteins made exclusively in thecytosol?______________
E. Entrance into which organelle(s) requires that proteinsunfold and snake through the
membrane? _________________
F. How do organelles maintain their position in the cytosol? Bespecific.
G. Give 2 advantages that eukaryotic cells gain by havingorganelles.
7. Which of the following statements are true regardingactin?
a. They are arranged in a
Related textbook solutions
Related questions
The vesicles that transport materials from donor membranes to acceptor membranes include:
| 1. | COP-II coated vesicles | |
| 2. | COP-III coated vesicles | |
| 3. | COP-I coated vesicles | |
| 4. | Clathrin coated vesicles |
2.5 points
QUESTION 11
During skeletal muscle contraction, the roles of ATP include:
| 1. | ATP is not required for forming the crossbridges between myosin and actin filaments. | |
| 2. | ATP is utilized as a structural linker to mediate the interaction between myosin and actin filaments | |
| 3. | Hydrolysis of ATP provides energy for myosin head to move along actin filaments and the binding of ATP to myosin head allows the detachment of myosin fromactin filaments. | |
| 4. | ATP binds to actin filaments to drive the power stroke of myosin head. |
2.5 points
QUESTION 12
One of the functions of smooth endoplasmic reticulum is
| 1. | Synthesizing steroid hormone in the endocrine cells of the gonad and adrenal cortex | |
| 2. | Serves as the starting point of the biosynthetic pathway | |
| 3. | Synthesizing secretory proteins | |
| 4. | Degrading cell debris |
2.5 points
QUESTION 13
The function of COP-II coated vesicles is mainly:
| 1. | Transporting materials from endosomes to Golgi complex | |
| 2. | Moving materials from the ER to the ERGIC and Golgi complex. | |
| 3. | Transporting materials from Golgi complex back to endoplasmic reticulum. | |
| 4. | Involved in receptor-mediated endocytosis. |
2.5 points
QUESTION 14
Which of the following processes is completed in rough endoplasmic reticulum?
| 1. | Chaperones help misfolded proteins fold correctly. | |
| 2. | Proteins that target to nucleus are synthesized in rough endoplasmic reticulum. | |
| 3. | O-linked glycosylation occurs in rough endoplasmic reticulum. | |
| 4. | Misfolded proteins are destructed in rough endoplamic reticulum. |
2.5 points
QUESTION 15
Kinesin is the motor protein that coorporates with microtubules. It has the following functions:
| 1. | It uses GTP as energy source. | |
| 2. | It can hydrolyze ATP and use the energy to drive cargo along microtubules from minus end to plus end. | |
| 3. | It has a globular head that can act as GTPase | |
| 4. | The tail of kinesin is more diverse and binds to cargo |
2.5 points
QUESTION 16
For correct segregation and trafficking, lysosomal proteins are tagged in the cis-Golgi with phosphorylated mannose residues, and then the tagged lysosomal enzymes are recruited to clathrin coated vesicles by mannose 6-phosphate receptors.
True
False
2.5 points
QUESTION 17
The process that specialized cells, such macrophages, engulf relatively large particles is:
| 1. | Called pinocytosis | |
| 2. | Called phagocytosis | |
| 3. | Mediated by COP-II coated vesicles | |
| 4. | Called autophagy |
2.5 points
QUESTION 18
Proteins synthesized on rough endoplasmic reticulum and smooth endoplasmic reticulum are imported co-translationally to their destinations
True
False
2.5 points
QUESTION 19
Treatment of cells with a drug that promotes microtubule disassembly disperses Golgi complex into separate stacks of membrane compartments, suggesting that:
| 1. | Golgi complex is made of microtubules. | |
| 2. | Microtubules are important for the organization of Golgi complex. | |
| 3. | Microtubules are transported into Golgi complex | |
| 4. | Golgi complex is composed of tubulin proteins |
2.5 points
QUESTION 20
Which of the following is correct about signal sequence of a secretory protein?
| 1. | It is an âaddress codesâ for protein trafficking pathways | |
| 2. | It is usually located at the C-terminus. | |
| 3. | It targets the ribosome and the protein that is being synthesized to endoplasmic reticulum. | |
| 4. | It is removed in Golgi complex. |
