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a) Can clones in a cDNA library contain 5′ UTR sequences? 3′ UTR sequences?

b) Would you be likely to find on average longer ORFs in cloned sequences from a genomic library or from a cDNA library? Explain.

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Bunny Greenfelder
Bunny GreenfelderLv2
28 Sep 2019

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Related questions

1. The lungfish Protopterus aethiopicus has a genome 38 timeslarger than that of humans. Most of the DNA in this species isnoncoding repetitive DNA. What type of library would allow you tocompare just the genes in the lungfish to the genes in humans?

Select one:

a. Transgenic library

b. Genomic library

c. cDNA library

d. PCR library

e. Knockout library

2.Gel electrophoresis can be used to separate DNA on the basisof

Select one:

a. nucleotide content.

b. Both a and b

c. the probe used.

d. size.

e. electrical charge.

3.Which of the following is FALSE of DNA sequences within a cDNAlibrary?

Select one:

a. They do not contain promoters.

b. They do not contain exons.

c. They do not contain introns.

d. They do not contain enhancers.

Are the following statements true or false? Please explain your answers.

1. Digestion of genomic DNA with AluI, a restriction enzyme that recognizes a four-nucleotide sequence, produces fragments that are all exactly 256 nucleotides in length.

2. To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used.

3. It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same tissue.

your friend has isolated a protein present in the cheek cells of all straight A seniors at your school. She says that this protein helps you remember everything you read and therefore will help you cut down on the number of hours needed to study for exams. She sequences the protein, which she calls geniuszyme and Designs a probe to isolate the gene that encodes it. To make sure she designs the probe correctly, she consults with the company that clone Factor 8. They have 100% confidence that her probe will work she also obtained a high-quality liver cdna library from the company and uses her probe to try to isolate the gene for geniuszyme. Unfortunately she has she is unable to isolate any clones.

A. what is the likely explanation for her failure

B. not to be discouraged comma your friend has obtained some genomic DNA isolated from the nuclei of liver cells and has made a genomic library from that DNA. Do you expect you will succeed and isolating the geniuszyme from this Library? Why or why

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