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A stain for bacteria flagella is very difficult and often does notshow much as is seen in this flagella preparation of a motilebacterium. Which of these species is most likely the motilebacterium on the slide?

Which bacteria could not metabolize Mannitol?

Why do we use a Gram-stain microbiology?

Which of the cultures would be least adaptive to varying oxygenconditions?

What is the requirement of a facultative anaerobe?

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Jean Keeling
Jean KeelingLv2
28 Sep 2019

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Related questions

16. All of the following are true about ear infections EXCEPT:

Middle ear infections (otitis media) often produce a puslike exudate (referred to as otitis media with effusion) due to infection by genera of Streptococcus, Haemophilus, or various species of anaerobes.

External auditory canal (otitis externa) infections are typically caused by Staphylococcus aureus or Pseudomonas aeruginosa.

After successful treatment of middle ear infections with antibiotics, sometimes tubes are inserted to prevent fluid accumulation, repeated infections, and hearing loss.

Pseudomonad infections are common in swimmers because these organisms are able to produce a soluble greenish-blue pigment.

Repeated ear infections in children often decrease markedly because as the child ages and the Eustachian tube changes shape and develops an angle that prevents most organisms from reaching the middle ear.

17. Why are colds difficult to treat and prevent?

Different cold viruses predominate during different seasons.

Because there are so many different types of rhino- and coronaviruses, each with different antigens, development and administration of vaccines are currently prohibitive.

Although some human interferons have been shown to block or limit rhinovirus infections, delivery to infection sites is difficult, and unwanted side effects can occur.

All of the above.

Both a and c.

18. Which of the following is NOT true about parainfluenza viruses?

Most infants by the age of 6 months have been exposed to and develop antibodies against all 4 parainfluenza viruses.

They cause rhinitis characterized by nasal, pharyngeal, and bronchial inflammation, primarily in children.

Parainfluenza virus infection can be prevented by vaccination.

They can cause noisy respiration (stridor) and acute obstruction of the larynx called croup.

The viruses are spread by direct contact or by large droplets.

19. In whooping cough, patients experience violent coughing episodes during the _________ stage and can have bluing of the skin or _________ due to lack of oxygen from massive mucus and bacterial airway blockage.

Paroxysmal; cyanosis

Catarrhal; pigmentation

Convalescent; osmosis

Pneumotic; hydrolysis

Primary; emesis

20. Penicillins have no effect on Mycoplasma pneumoniae because:

Mycoplasmas are viruses

Mycoplasmas possess beta lactamases

Mycoplasmas are too small

Mycoplasmas are eukaryotes

Mycoplasmas lack cell walls

21. How many cases of tuberculosis are reported globally each year?

10

10,000

100,000

3 million

10 million

22. Mycobacteria are difficult to Gram stain, and are termed “acid-fast” due to their:

Ability to survive in acidic conditions

Resistance to drying

Thick, waxy cell walls

Resistance to sunlight

Lack of a peptidoglycan layer

23. Which of the following is true about pathogenic mycobacteria?

They have a long generation time of 12 to 18 hours.

They are obligate anaerobes sensitive to small amounts of oxygen.

They are highly resistant to drying and can remain viable in dried sputum 6 to 8 months later.

Both a and b.

Both a and c.

24. Clinical symptoms of tuberculosis are primarily due to:

Host inflammatory response

Mycotoxins

Endotoxin

Mucus production

Exotoxin

25. What characteristic of Coxiella burnetii, the causative agent of Q fever, allows it to survive for up to 2 years?

Metachromatic granule

Exotoxin

Endospore

Hemagglutinin

Cilia

26. The normal microflora of the heart includes species of:

Gram-positive bacteria

Gram-negative bacteria

Fungi

Viruses

None of these

27. Which of the following is commonly directly responsible for causing septic shock today?

Bacterial hemagglutinins

Bacterial endotoxins

Bacterial exotoxins

Fungal aflatoxins

Bacterial neurotoxins

28. All of the following are symptoms of septic shock EXCEPT

Shock

Lymphangitis

Chills

Fever

Collapsed blood vessels

29. What is the most likely explanation of how Streptococcus pyogenes causes rheumatic fever?

Strep throat infections migrate down to the heart.

Endotoxin production causes septicemia.

Vegetation followed by fibrin deposition.

Cross-reactivity of an antibody for a Streptococcus pyogenes antigen to a heart cell antigen.

It is an opportunistic infection triggered by coronary artery disease and atherosclerosis.

30. Bacterial endocarditis is an infection and inflammation of the lining and valves of the heart and occurs as a result of transient bacteria attaching to fibrin from exposed collagen fibers of damaged valvular surfaces. True or false?

Part III – The Physician
June 5th
ree weeks had passed. e swelling was still present and it had become even more di cult for Margo to move
her nger. In addition, the amount of uid oozing from the wound had increased, suggesting that the medication hadn’t worked. It was now time to visit a local physician. Following the advice of a neighbor, Margo set up an o ce appointment with Dr. Chan, an internist. After the usual battery of questions, Dr. Chan sent in the nurse to collect a specimen from Margo’s nger. Dr. Chan mentioned to Margo that the specimen would be sent o to the regional hospital laboratory for analysis and that he would provide her with another prescription for Ke ex, which she should continue to take until test results came back.
June 15th
e test results arrived in Dr. Chan’s o ce on June 15th and were as follows: • No yeast or fungal elements were noted
• Gram-stain reaction: no organisms seen
• Culture of anaerobes and aerobes @48 hours yielded no growth
• Enrichment culture results: moderate growth on day 5 of an acid-fast bacterium (AFB). o Growth identi ed as Mycobacterium fortuitum by the Florida State Laboratory.
Armed with a microbial identi cation, Dr. Chan prescribed Doxycycline and Bactrim to be taken for four months. Dr. Chan also mentioned that the isolated organism was known to be found in tap water. Consequently, Margo planned to ask her condo community why an organism that could cause so much pain would be living in the water that was coming out of her faucets. And although she was annoyed to learn of the source of the organism, at least she could take some comfort in the fact that she nally had an accurate diagnosis and that by taking her medicine she could fully recover from her injury and start to enjoy her new home.
Questions
1. Compare and contrast aerobes and anaerobes. Why were no microbes noted upon aerobic and anaerobic culture?
2. Investigate the properties and characteristics of M. fortuitum. Where is this organism typically found (reservoirs)?
3. Why must the acid fast stain be performed to identify organisms of the genus Mycobacterium?
4. Why did Dr. Chan prescribe two antibiotics rather than only one?
5. How does doxycycline kill bacteria (cellular target)?
6. Why were the antibiotics prescribed for an extended period (4 months)?
7. No organisms were noted upon Gram staining, yet the enrichment culture yielded M. fortuitum after several days. Provide an explanation.

5. Some bacteria live in deep sea vents where no light penetrates, yet are still capable of producing their own reduced carbon (e.g. fixing carbon dioxide). These organisms are classified as

(A) photoheterotroph (B) photoautotroph

(C)chemoheterotroph (D)chemoautotroph

(E) organotroph

6. Nitrogen fixation is the conversion of

(A) ammonium ion to nitrogen gas. (B) nitrogen gas to ammonium ion.

(C)nitrate ion to nitrogen gas. (D)ammonium ion to nitrate ion.

(E) nitrate ion to ammonium ion.

7. Which of the following is true of complex media?

(A) Bacteria grow faster on them than on synthetic media.

(B) All the chemical components and their concentrations are known.

(C)Complex media are the most difficult of all to prepare.

(D)Complex media can help determine the metabolic capacity of an organism.

(E) They are also called minimal media.

8. Which of the following types of growth media contains blood?

(A) minimal media (B) complex media

(C)defined media (D)enriched media

(E) selective media

9. The bile salts and crystal violet in MacConkey lactose plates prevent the growth of Gram-positives. These additives make the MacConkey plate a

(A) complex medium. (B) defined medium.

(C)minimal medium. (D)selective medium.

(E) differential medium.

10. You discover a new bacterial efflux pump that moves an uncharged toxin out of the cell using the proton motive force as the energy source. This new transporter can be classified as an

(A) electrogenic antiporter (B) electrogenic symporter

(C)electroneutral antiporter (D)electroneutral symporter

(E) none of the above

11. Which of the following involves nitrate as a terminal electron acceptor?

(A) photosynthesis (B) aerobic respiration

(C)fermentation (D)anaerobic respiration

(E) glycolysis

12. Which of the following is least likely to use glucose (an organic molecule) as a source of electrons?

(A) lithotrophy (B) organotrophy

(C)aerobic respiration (D)anaerobic respiration

(E) heterotrophy

17. Which of the following nutrient uptake systems does not require cellular energy?

(A) facilitated diffusion (B) coupled transport

(C)ABC transporters (D)group translocation

(E) All of the above require energy expenditure by the cell

18. What is the energy source for ABC transporters?

(A) proton motive force (B) NADH

(C)phosphoenolpyruvate (PEP) (D)ATP

(E) No energy is required for ABC transport.

19. Which of the following is true of all biofilms?

(A) They only contain a single species of bacteria.

(B) Biofilms can form on a range of biotic or abiotic surfaces.

(C)Cells in a biofilm are dormant until the bacteria leave the biofilm.

(D)Biofilms are composed of a single monolayer of EPS.

(E) Biofilms are not found in or on the human body.

20. The heterocyst cells of cyanobacteria are specialized for

(A) photosynthesis (B) gliding motility

(C)nitrogen fixation (D)carbon fixation

(E) antibiotic production

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