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I need help on designing lab protocol for biology on the -Effect of pH on enzyme activity. The enzyme we areusing for this lab protocol is Carbonic Anhydrase.How would I start the purpose of this lab, the procedure & whatcould controls be for this lab?

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Collen Von
Collen VonLv2
29 Sep 2019

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Related questions

A. extraction of amylase from barley seeds

B. altering enzyme concentration to determine its effect on aconstant concentration of substrate

C. Determining the effect that ph has on enzyme containingreaction systems

D. determining the effect that temperature has on enzyme-containing reaction systems

the intro, objective, materlals, procedure, results, disscusion,conclusion.

This is the first lab report for me anyone have backgroundshould i do it separate,, any formal for this lab will be great

Design an experimentin which you will test the effect of an acidic fluid on enzymaticactivity. (Recall: enzymes are proteins.) To complete this project,it may be useful for you to review the Scientific Method Tutorial (found the CourseContent section of the classroom under the Science Learning Centerlink) and the Scientific Method lab (Lab 1), so that you canbetter understand how to design an experiment. It may also behelpful for you to review Lab 4 (Enzymes). As you review Lab 4, youwill be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).

As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must alsothoroughly explain how the addition of the acidic fluid impactedthe overall reaction process (this information will help you toanswer question 4).

Hint: Keepin mind that the acid will change the environmental conditions ofthe experiment (for example, a low pH value could change the shapeof the active site on the enzyme protein), without directlyparticipating in the reaction.

please address the following:

1. What question are you asking?Clearly state your hypothesis.

2. Design an experiment. Provide adetailed account of the materials and methods used to conduct theexperiment. Also include the methods for data collection andanalysis.

3. Conduct the experiment and recordyour results below. What did you observe? Which samples showedbubbling?

4. Use your knowledge about factorsthat effect enzymatic activity to interpret your results. It may benecessary for you to refer to your lab manual and/or otherresources. What effect did the acid have on the enzyme?

5. Was your hypothesis supported? Whatis your conclusion? Explain your answer.

6. Cite all references used tocomplete your Final Applied Project.


Can you help me with my Final Applied Lab Project forBiology103?

How can I create an experiment testing the effects of low pH onenzyme activity?

Help! I’m supposed to do an experiment, but I’m kind ofconfused. I have five test tubes, and I’m supposed to be testingthe effects of low pH on enzyme activity. I’ve chosenAmylase/banana as my enzyme and starch/potato as my substrate. (Ialso have left over starch liquid and IKI.) I was going to test thedifferent pHs by adding water (7), draino (14), soda (4), banana(5) and soda (4) to 1 cubic centimeter of a potato in each tube. Iguess I’m just not sure if I’m doing it right.

The independent variable influences the change in my dependentvariable. Is my dependent variable the potato? My independentvariable would be the water, draino, soda, banana and soda. Whatwould my control be? Thank you for your help.

Am I supposed to put 1 cubic centimeter of potato in each test tubeand then only put the banana in one test tubes with the potato orget rid of the potato and only use the banana in each test tube?Sorry, I'm confused.

Design an experiment in which you will test the effect of anacidic fluid on enzymatic activity. ID the enzyme, the substrate,the acidic fluid used as treatment, the control treatment and themethod of measuring enzyme activity, as well as explain yourexperimental protocol. You must also thoroughly explain how theacidic fluid impacted enzyme activity based on the results fromyour own experiment as well as knowledge of enzymes and pH.

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