You start with a 75 mM pNPP solution. You add 350 mL of this solution to prepare a 3500 mL master mix. You then add 10 uL of the master mix cocktail and dilute it to 100 uL total volume. The final concentration of the pNPP is:
a. 7.5 mM
b. 75 uM
c. 750 uM
d. 750 mM
You start with a 75 mM pNPP solution. You add 350 mL of this solution to prepare a 3500 mL master mix. You then add 10 uL of the master mix cocktail and dilute it to 100 uL total volume. The final concentration of the pNPP is:
a. 7.5 mM
b. 75 uM
c. 750 uM
d. 750 mM
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Hi, I really need up fiuring out how to do this. I'm doing a1:10 ratio dilution. I know it's regular chem. lab but I can'tremember. Equation and help please. Need by tonight.
Use 0.2 ml PCR tubes for these reactions. The thin wallfacilitates heat transfer. Be careful to not crush the tubesbetween your fingers. There is not much writing surface on thesetubes so it is best to create a log in your notebook and thennumber the tubes accordingly.
Reagent | Stock Conc | Final Conc | Dilution | Volume per Sample | Volume per Master Mix (N+1) |
Template DNA | 5 ul | ||||
5X GoTaq Flexi Buf | 5X | 1X | |||
dNTP Mixture | 10 mM | 0.2 mM | |||
MgCl2 | 25 mM | 2.5 mM | |||
D1S80 Fwd Primer | 10 uM | 0.5 uM | |||
D1S80 Reverse Primer | 10 uM | 0.5 uM | |||
GoTaq Hot Start Polymerase | 5U/ul | 1.25U | |||
MGW (Molecular Grade Water) |
