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Next, obtain reagents for your standard curve by mixing BSA samples differing in protein concentration (a stock of BSA has been prepared for you at a concentration of 2 mg/ml). To do this, label a set of microfuge tubes from 1-6. Then, follow the chart below to fill the tubes with appropriate amounts of BSA and water. Be sure to thoroughly mix tubes. Determine the final BSA concentration using the equation C1V1 = C2V2

Tube Volume of Water Volume of BSA (Stock) Final BSA Concentration

1 10 ul 10 ul ____ ug/ml

2 12.5 ul 7.5 ul ____ug/ml

3 15 ul 5 ul ____ug/ml

4 17.5 ul 2.5 ul ____g/ml

5 19 ul 1.0 ul ____ug/ml

6 20 ul 0uL 0 ug/ml (blank)

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Elin Hessel
Elin HesselLv2
28 Sep 2019

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