Question

Please answer the following multiple part question:

a) The RE HhaI cuts the recognition site 5’GCGC3’. The probability of having a cutting site for this enzyme is about one in every __________bp of genomic DNA.

A. 100

B. 250

C. 1000

D. 10,000

b) The RE HinD III recognizes/cuts the site 5’AAGCTT3’. If you cut a 100,000 bp DNA fragment with the enzyme, you expect about _________ DNA fragments.

A. 15

B. 25

C. 35

D. 45

c) Methylation-sensitive REs _________.

A. do not cut DNA if the recognition site is methylated

B. cuts DNA only if the recognition site is methylated

C. shows more star activity than typical REs

D. lacks any isochizomers

d) DNA ligation is __________ (only the most appropriate answer will be scored)

A. cross-linking two molecules of DNA

B. cutting DNA (severing a phosphodiester bond)

C. linking two DNA molecules by any given bonds D. attaching two DNA molecules by restoring the phosphodiester bonds

e) RFLP is often used in human identification through DNA finger printing. RFLP is largely based on ___________.

A. mutation in the RE cutting sites

B. sequence-specificity of the type II REs

C. mutation of the genomic DNA in general

D. mutations in the RE recognition/cutting site and sequence-specificity of REs

f) RFLP pattern of the GAPDH gene of two siblings appeared different but both are healthy and well. It is most likely because ______.

A. The restriction enzyme used cuts DNA of different individual at different cut sites

B. One of the siblings has a mutation in the gene

C. The GAPDH gene is polymorphic