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13 Oct 2018

By what two different mechanisms does methylation of gene promoter regions cause silencing?

What is the role of DNA methylation in regulating transposons (Transposable Elements, TE’s; ‘jumping genes’)?

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Jarrod Robel
Jarrod RobelLv2
14 Oct 2018

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Related questions

• Identify the mechanisms for gene expression control in the lac and trp operons

• Explain what transcription factors and regulatory proteins are and how they regulated gene expression in eukaryotes.

• Describe how chromatin structure (DNA methylation and histone modification) can affect gene expression

• Explain the process of protein degradation and its purpose

• Contrast the mechanisms regulating gene expression depending on when they act on: 1) regulating transcription, 2) posttranscriptionally (regulating the mRNA and protein synthesis) and posttranslationally (regulation of protein degradation)

DNA Methylation

A. Name two mechanisms by which it inhibits gene expression

B. How can it activate gene expression?

C. How is methylation passed from one cell to its daughter?

D. How does methylation from the mother and father affect expression of the IGF2 gene?

E. When does imprinting get erased during the life cycle and when does it get established again? Is it completely erased?

Can methylation of nucleotides play a role in DNA replication?

If so, what role does it play?

Removal of methyl groups from nucleotides is concurrent with the conversion of dNTPs to dNMPs during DNA synthesis.

Lack of methylation on the synthesized DNA strand can be recognized by repair enzymes, thus permitting selective base pair mismatch repair.

Lack of methylation on the parental DNA strand can be recognized by repair enzymes, thus permitting selective base pair mismatch repair on the newly synthesized strand.

Methylation does not play a role in replication.

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