How does lactose control its metabolism? Use the lac system from E. coli as an example
For unlimited access to Homework Help, a Homework+ subscription is required.
1. You have discoveredan E. coli mutant that expresses a lac operon capprotein that is unable to bind cAMP. If you grew that mutant inglucose, lactose, IPTG, and glucose and lactose how would the?-galactosidase activity differ from an E. coli straincontaining a functional lac operon? What would happen if youtested an E. coli strain that contains a CAP protein thatcan bind DNA even in the absence of cAMP?
A bacterial cell has a mutation in LacY gene. Based on the information you know about lac operon system, how will this impact the lactose metabolism?
The expression of the lac operon in an E. coli cell missing the gene that encodes for the lac repressor will:
a. increase when lactose is present
b. decrease when lactose is present
c. decrease when glucose is present
d. increase when glucose is present