A bacterial cell has a mutation in LacY gene. Based on the information you know about lac operon system, how will this impact the lactose metabolism?

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Jamar FerryLv2

16 Jul 2019

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When (glucose, lactose) ___________ levels inside a bacterial cell are low, cyclicAMP levels are (high, low) __________. This is because metabolizes sugars (activate, inhibit) ___________ the enzyme adenylate cyclase, and (activate, inhibit) __________ the enzyme cAMP phosphodiesterase, causing cyclic AMP levels to rise. Consequently, the CAP protein is (Activated, inhibited, unaffected) ___________ by the cyclic AMP and binds to the lac promoter region, and the CAP protein acts like a sigma factor recruiting (DNA, RNA) ___________ polymerase to the promoter. This establishes condition that may lead to the (transcription, replication) __________ of the lac operon if (allolactose, glucose, lactose) _____________ is available to bind to (lac repressor, lacZ, lacY, lacA) _________ protein keeping this protein from binding to the (lacO, lacZ. lacY, lacA) ___________ gene.

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Mention the components of bacterial transcriptional switches. The Lac operon is regulated by both activator CAP and Lac repressor. A scientist introduces a mutation in the gene encoding the activator CAP, making this allosteric protein insensitive to glucose levels. What outcomes do you expect if you culture the mutated bacteria in a culture medium with glucose and lactose. Give your explanation based on the mechanism of the Lac operon.

In the following points, I have summarized Lac operon in Ecoli. I need to check if this is on the right track? and where does 'cleavage of disaccharides' fit into this picture?

Lactose is a disaccharide and the bacteria need specific genes to manage catabolism

1) The lacl promotor gene synthesis the repressor protein which binds to the operator gene stopping the RNA polymerase from coding the lac operon genes

2)But when lactose is present, it needs the specific genes to catabolise lactose

3) So, When allolactose (a derivative of lactose)is present, it binds to the repressor protein, the repressor releases from the operator gene

4) This allows the RNA polymerase can attach to the DNA control region

5) And drive down the operon for transcription and induction of the enzyme lacZ(beta-galactosidase) lacY and lacA which breaks lactose down into glucose and galactose

6) When the lactose is all gone, the repressor returns to the operator in a feedback loop system