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14 Nov 2018

A cell has a membrane potential of -90 mV. The concentration of Cl- is 4 mM in the ICF and 115 mM in the ECF. The equilibrium potential for Cl- is -70 mV. What would be the direction of the electrochemical driving force for Cl-?

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Sixta Kovacek
Sixta KovacekLv2
16 Nov 2018
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Related questions

1. You decide to measure the Vm of the membrane below to verify your calculation. You place an axon in a dish containing a solution that matches the table above. Next you put your reference electrode and the recording electrode into the solution. What do you read on the volt meter?

2. Now you puncture the axon membrane with the recording electrode and the voltmeter reads -62.1 mV. Is this different from your calculated Vm? Explain why it is different.

Ion ICF (mM) ECF (mM) Permeability (p)
K+

150

 15 110
Na+ 25 200 1.5
Cl- 10 150 15

You are observing a cell at equilibrium with respect to the potassium cation (K+). The cell is in a bathing solution of 1 mM potassium, and you measure the membrane potential of the cell to be -118 mV. What is the internal potassium concentration? show steps

100 mM 10 mM 0.01 mM 1 mM 0.1 mM

Assume ionic concentrations in the nervous system of:

[K]o = 4 mM

[K]i= 155 mM

[Na]o= 145 mM

[Na]i= 12 mM

7. Use the GHK equation (ignoring any contribution by Cl or the Na/K pump), and assume RT / zF = 58 mV, to calculate the RMP of a cell when the gK to gNa ratio is:

i) 10:1?

ii) 50:1?

iii) 100:1?

8. Which of the above permeability ratios most closely represents the membrane of a neuron at rest (assuming a cell at rest has an RMP of -70 mV)? Why? [2]

9. Which of the above permeability ratios most closely represents the membrane of a glia cell at rest? Why? [2]

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