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13 Apr 2018
1. You decide to measure the Vm of the membrane below to verify your calculation. You place an axon in a dish containing a solution that matches the table above. Next you put your reference electrode and the recording electrode into the solution. What do you read on the volt meter?
2. Now you puncture the axon membrane with the recording electrode and the voltmeter reads -62.1 mV. Is this different from your calculated Vm? Explain why it is different.
Ion ICF (mM) ECF (mM) Permeability (p) K+ 150
15 110 Na+ 25 200 1.5 Cl- 10 150 15
1. You decide to measure the Vm of the membrane below to verify your calculation. You place an axon in a dish containing a solution that matches the table above. Next you put your reference electrode and the recording electrode into the solution. What do you read on the volt meter?
2. Now you puncture the axon membrane with the recording electrode and the voltmeter reads -62.1 mV. Is this different from your calculated Vm? Explain why it is different.
Ion | ICF (mM) | ECF (mM) | Permeability (p) |
K+ | 150 | 15 | 110 |
Na+ | 25 | 200 | 1.5 |
Cl- | 10 | 150 | 15 |
Bunny GreenfelderLv2
16 Apr 2018