What organic molecule does Benedictâs detect and how (what specific functional group)? What indicates a positive reaction after adding Benedictâs? What specifically do you have to do while performing this test?
What organic molecule does Iodine detect? What indicates a positive reaction?
What organic molecule does Sudan IV detect and why (what specific functional group)? What indicates a positive reaction?
What organic molecule does Biuret detect and why (what specific chemical component)? How is this different from Ninhydrin (what specific functional groups does this detect)? What indicates a positive reaction for each reagent?
Know how to apply these to determining the characteristics of an unknown (which would be provided)
What organic molecule does Benedictâs detect and how (what specific functional group)? What indicates a positive reaction after adding Benedictâs? What specifically do you have to do while performing this test?
What organic molecule does Iodine detect? What indicates a positive reaction?
What organic molecule does Sudan IV detect and why (what specific functional group)? What indicates a positive reaction?
What organic molecule does Biuret detect and why (what specific chemical component)? How is this different from Ninhydrin (what specific functional groups does this detect)? What indicates a positive reaction for each reagent?
Know how to apply these to determining the characteristics of an unknown (which would be provided)
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Life Chemistry LabReport
- Elements of Living Organisms
- Name the five most abundant elements found in the humanbody.
- Cells organize into tissues which work together to form
- What is the difference between an atom and an element?
- Molecules
- Is glucose an atom or molecule? What are the atoms inglucose?
- How many bonds can oxygen form?
- Is C6H12O6 a molecular orstructural formula? What is the name of this molecule?
- Based on lab, Section II, draw and label a ball and stickrepresentation of a carbon dioxide, water and ethyl alcoholmolecule. Use circles to designate atoms and lines for bonds, thenindicate the chemical symbol for each atom. Sign, date and preparean image of your drawing and include it with this lab report.
- Molecular Make Up of Living Organisms
- Name the four groups of organic molecules.
- _______ are the most abundant organic molecules in livingorganisms.
- Name a polysaccharide, monosaccharide, protein and alipid.
- Go to the lab, Section 3, Exercises 2, 3, 4 and 5 to completethe following table:
Type of Organic Molecule | Reagent | Sample Tested | Positive Color | Negative Color |
Carbohydrate (starch) | Iodine | |||
Sugar | Benedictâs solution | |||
Lipid (fat) | Sudan IV | |||
Protein | Biuret |
- Go to the lab, Section 3, Exercise 6 to locate starch in potatocells. Describe the microscopic appearance of starch in terms ofcolor and location within the cells.
- Measurement of pH
- What substance has an equal number of hydrogen ions(H+) and hydroxide ions (OH-)?
- What is the pH of the above substance?
- How does the addition of hydroxide ions (OH-) affectthe pH of your stomach?
- Go to lab, Section 4, Exercise 7 to test the pH of commonsolutions. Which solution tested had a pH closest to neutral? Whichsolution was most acidic?
Summary Questions
- What is the smallest subunit of carbohydrates?
- Which reagent could be used to test for diabetes?
- A member of your family has a high blood cholesterol count. Yousee âfat freeâ crackers in the grocery store and decide to test theproduct for the presence of lipids. How would this testproceed?
- A family member has been cautioned about eating foods high insugar. Explain how a test could be run for the presence ofsugar.
- Explain how to test for the presence of protein in milk.
- A friend complains of stomach acidity. Which substance testedmight safely help relieve your friendâs symptoms?
- Explain how solutions are classified as acids or bases. Then,give an example of a weak acid.
Experiment 1: Testing for Proteins
Data Tables
Table 1: Testing for Proteins Results
Sample | Initial Color | Final Color | Is Protein Present? |
---|---|---|---|
1. Albumin Solution | |||
2. Gelatin Solution | |||
3. Glucose | |||
4. Water | |||
5. Unknown |
Post-Lab Questions
Record your hypothesis about what will happen when Biuret solution is mixed with the solutions from test tubes 1, 2, 3, and 4 here. Be sure to use scientific reasoning to support your hypothesis.
Write a statement to explain the molecular composition of the unknown solution based on the results obtained during testing with the Biuret solution and each sample solution.
Diet and nutrition are closely linked to the study of biomolecules. How should you monitor your food intake to ensure the cells in your body have the materials necessary to function?
There are other types of reagents used to determine what type of biomolecule a substance is. For example, copper ions present in Benedictâs reagent reacts with the free end of any reducing sugars, such as glucose, when heated. Originally blue in color, these copper ions are reduced by the sugar, and produce an orange-red colored precipitate. Alternatively, iodine-potassium iodide (IKI) may also be used when working with starch. IKI contains special tri-iodine ions which interact with the coiled structure of a starch polymer. Prior to a reaction, the IKI displays a yellow-brown color; however, after reacting with starch, a dark purple or black color is presented.
The molecule pictured below produced a blue color when tested with Benedictâs reagent, a yellow color when tested with IKI, and a violet color when tested with Biuret reagent. Based on the structure shown below and these chemical results, what kind of biomolecule is this?