BIO 209 Lecture Notes - Lecture 16: Lysosome, Exocytosis, Cholesterol
6 Dec 2016
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Document Summary
Vesicular transport, donor and target compartments, anything in the donor compartment is trapped in that vesicle, vesicle then transports to the target compartment where it fuses and releases its contents. How are directional pathways determined: vesicles are coated with specific proteins. Proteins direct the vesicle: clathrin coated- involves endocytosis, clathrin also allows bending, copi- comes from the golgi, copii- comes from the er. Phospholipid content inside vesicle also directs vesicles: phospholinsitides- serves as the binding sites. Regulatory g proteins: gtp binding proteins, gdp bound= inactive, gtp bound= active state that triggers a downstream response. Exchange reaction activates g proteins: upstream activating signal removes gdp and replaces it with gtp, hydrolysis- the self-regulating reaction. Takes bound gtp and removes an inorganic phosphate, causing it to revert back to its inactive gdp state: rabs- site specific gtpases that regulate coat assembly. Vesicles to target membrane vsnare- on vesicles tsnare- on target membrane.
