BIO315H5 Lecture Notes - Lecture 12: Copi, Hydrolysis, Gtpase
Document Summary
Coated vesicles: trafficking between compartments uses coated vesicles, vesicles are enclosed compartment with luminal contents and surrounding membrane, for them to bud, need specific proteins that can coat the membrane. Rab proteins guide vesicle targeting: a monomeric g protein, many diff rab proteins that are associated with diff membrane compartments. If you play around with the rate of gtp hydrolysis, you can change what happens hydrolyze, slow but predictable- gives the vesicle just enough time to pinch off the membrane. If increase the rate of gtp hydrolysis, the vesicles will not have been fully formed before the protein can no longer associate, coat proteins leave and you cannot finish the formation of that vesicle (vesicle formation is impaired) If you prevent gtp hydrolysis, you cant strip the coat of the vesicle. If have specific vsnare on a specific compartment, it is only going to recognize a specific pairing of tsnares that is only found in 1 compartment.
