BCH 2333 Lecture Notes - Lecture 15: Allosteric Regulation, Sigmoid Function, Enzyme Inhibitor

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It is the binding of some ligand either to the active site or the distant site. As soon as there is too little, atcase starts making more: when you look at the curve, it is a sigmoid. It is an r-state and a t-state curve, depending on the cooperativity. h. In the presence of high concentrations of ctp, atcase can remain inactive: the regulatory domain can bind to both atp and ctp. When atp binds, it accelerates the production of ctp. Ctp stabilizes the t-state and atp stabilizes the r-state: atp is required down-stream in the production of ctp, we have catalytic trimers with 2 regulatory units. Two of them come together so we end up with 6 catalytic subunits: ctp binding will bring the regulatory units into conformation. Atp binding causes the catalytic sites to rotate, it basically opens up: calculation for allosteric constant will give you the ratio of t:r state.

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