BCH 2333 Lecture Notes - Lecture 15: Phosphofructokinase, Glycogen Phosphorylase, Michaelis–Menten Kinetics
21 Mar 2016
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Fairly long pathway inal product is ctp. Control how much material will be processed by the pathway? o o o. Pathway will slow down when lots of product is formed. Not enough product will result in a non-inhibited pathway. Cells usually have concentraion limits for a certain product. Need to bind to the catalyic subunit domains. Atp and ctp (not substrates) o o o. More r state present means more acive; more t-state means less acive enzyme. Highly cooperaive - all subunits change conformaion together! T state is more stable, inacive (when nothing is bound to it) Catalyic domain is more stable, t:r = 200:1 o o. R state brings substrates closer for the rxn: Substrate binds to the t state which causes a conformaional change. When substrate is binded (to r), r state is more stable. Acivator will bind preferenially to the r state o. Inhibitor will bind preferenially to the t state o.
