GEN-3000 Study Guide - Midterm Guide: Antimicrobial Resistance, Prophage, Wild Type

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4 Oct 2016
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Chapter 8: advantages of bacteria/viruses, rapid reproduction, many progeny produced, small genomes, easy growth in laboratory, medical importance, haploid, prototroph vs. auxotroph, prototroph wild type bacteria than can synthesize all compounds needed for growth from simple ingredients. Minimal media: auxotroph - mutant strain that lacks one or more enzymes required for metabolizing nutrients - will grow on supplemented media b. i. Will live on complete media for a little while b. ii. If you give it what it"s mutant for it will survive b. iii. Have to be on selective media with their component: bacterial characteristics, haploid, single circular chromosome, not all but most, asexual reproduction, live in a media what they need to survive d. i. Minimal media: horizontal gene transfer abilities e. i. Donor cell with plasmid makes bridge (pilus) and travels across: f factor a. i. This special plasmid that makes the bridge a. ii. Insertion sequences that makes the plasmid an episome: different f cell types b. i.