GEN-3000 Lecture Notes - Lecture 8: Auxotrophy, Transfer Dna, Plasmid
21 Feb 2017
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Chapter 8 - bacterial and viral genetic systems. Single circular chromosome - 4. 6mb, 4000 genes. Most bacteria possess a single circular chromosome. Serial dilutions so we can get a single colony all by itselfs; shows different phenotypes. Wild type bacteria that can synthesize all compounds needed for growth from. Mutant strain that lacks one or more enzymes required for metabolizing nutrients. Will grow on supplemented media (auxotroph"s are mutants like x men . Medium is food source for bacteria can help us figure out genotype. Gives bacterial cells everything they could possibly need to survive and grow. Direct transfer of dna from one bacterium to another (donor or recipient only) Bacterial viruses (phages) carry dna from one bacterium to another. Dna exchange and reproduction are not coupled in bacteria. All dna exchange - switch out what we brought in with what we have. Auxotrophs mix together and we get autotrophs. Filter between them has holes and medium.
