1. In order to have 99% of the glycine in its –NH3+ form, what must the numerical relation be between the pH of the solution and the pKa of the amino group of glycine?

For an α-helical polyalanine peptide 45.00 residues in length, calculate the melting temperature in °C. Tm= °C

Calculate the melting temperature of a 45.00 amino acid α-helical peptide if that peptide contains all alanines and a single internal glycine residue. Tm= °C

Calcuate the melting temperature if the 45.00 amino acid α-helical peptide contains 5.00 internal glycine residues and a glycine at the N-terminus of the helix. Tm= °C

Calculate the melting temperature for a 45.00 amino acid polyhistidine peptide at physiological pH in a 310-helix. Tm= °C

Consider a glycinergic neuron and a glutamatergic neuron. Doesthe mechanism of neurosecretion differ between these two types ofneurons?

answers are either yes or no

1) 4L1X Transfer Buffer: 25mM Tris, 192mM Glycine, 20%(v/v) Methanol, 0.1%(w/v) SDS

Calculate the volume amount of 10X Transfer stock solution to dilute to 1X concentration in 1L. Calculate the volume amount of methanol to make a final concentration of 20% (v/v) and the volume amount of 10% (v/v) SDS to make a final concentration of 0.1% (v/v)