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1) 4L1X Transfer Buffer: 25mM Tris, 192mM Glycine, 20%(v/v) Methanol, 0.1%(w/v) SDS

Calculate the volume amount of 10X Transfer stock solution to dilute to 1X concentration in 1L. Calculate the volume amount of methanol to make a final concentration of 20% (v/v) and the volume amount of 10% (v/v) SDS to make a final concentration of 0.1% (v/v)

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Deanna Hettinger
Deanna HettingerLv2
28 Sep 2019

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Related questions

To collect DNA you need to lyse the cells with a lysis buffer. To make this you first prepare stock solutions of NaCl (4M), Tris (1M, pH 8), 10% SDS (w/v) and Na2 EDTA (dehydrate)(0.5M). How would you make 100 mL of each stock solution? Note: Look up molecular weights.

To collect DNA you need to lyse the cells with a lysis buffer. To make this you first prepare stock solutions of NaCl (4M), Tris (1M, pH 8), 10% SDS (w/v) and Na2 EDTA (dehydrate)(0.5M). How would you make 100 mL of each stock solution? Note: Look up molecular weights.

10. When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose will you require? [you will make this later, not on Solution day] When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains: • glycerol, which helps the samples sink into the sample wells of the gel; and • tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis. The recipe for 6X loading dye is as follows: • 0.25% bromophenol blue (w/v) • 0.25% xylene cyanol (w/v) • 30% glycerol in water (v/v)

11. Provide a recipe for 10 ml of 10X loading dye, using the information above. (Important tip: Remember that you have already made 30ml of a 50% stock solution of glycerol. I recommend determining the concentration of glycerol required for 10X loading dye, then figuring out how much of your 50% glycerol stock will be needed to make 10ml of 10X loading dye.)

Solution to make

Exact pH of final solution (if appropriate)

Final container for solution

Completed (Y or N)

100 ml of 0.9% (w/v) NaCl

100 ml of 1.0M Tris, pH 7.6

40 ml of 50X TAE

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