Pagalan ng may-akda

I need a concentration of 10 ng/ml but my current concentrationis 156 ng/ml. How would I go about converting that?

I need help with diluting a template for a PCR reaction.

I need to use 50 ng of template and I have template concentration of 119.4 ng/ul, and I am asked to dilute that to 50 ng/ml. My question is how many ul of template do I need to add?

How can I improve my Gel Extraction yields. We use the standardprotocol from Qiagen, gel extraction, dissolve in QG buffer at 42Cand purify via anion exchange columns. However, with 500 ng weultimately get 100 ng for a 20% yield. I was curious what else hasbeen tried for gel extraction.

We also add IPA and NaOAc to the dissolved mixture prior to thecolumn purification.