BamHI cuts the sequence 5'-G|GATCC 3-'. Which of the following sequences would not be recognized by this enzyme?
5'-AGGATCCGTA-3'
3'-CCTAGGATC-5'
5'-AGCGGATCC-3'
3'-TCTTAAG-5'
3'-TCCATGGG-5'
BamHI cuts the sequence 5'-G|GATCC 3-'. Which of the following sequences would not be recognized by this enzyme?
| 5'-AGGATCCGTA-3' | ||
| 3'-CCTAGGATC-5' | ||
| 5'-AGCGGATCC-3' | ||
| 3'-TCTTAAG-5' | ||
| 3'-TCCATGGG-5' |
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Related questions
What is the expected frequency of the restriction site 5'-GGCC-3' in a genome?
| A | Once every 4 nucleotides | |
| B | Once every 16 nucleotides | |
| C | Once every 64 nucleotides | |
| D | Once every 128 nucleotides | |
| E | Once every 256 nucleotides |
BamHI cuts the sequence 5'-G|GATCC 3-'. Which of the following sequences would not be recognized by this enzyme?
| A | 5'-AGGATCCGTA-3' | |
| B | 3'-CCTAGGATC-5' | |
| C | 5'-AGCGGATCC-3' | |
| D | 3'-TCTTAAG-5' | |
| E | 3'-TCCATGGG-5' |
We have four DNA sequences that we need to digest (cut up), and have 6 restriction enzymes in stock in our lab. We want to find out which restriction enzyme we can use on each sample.
For each sequence, please indicate which restriction enzyme(s) would cut within each sequence. Each sequence may be cut by one, more than one, or no restriction enzymes.
Following the sequences are two questions. Please choose from the same list of enzymes to indicate the correct answer to these questions.
| Restriction enzyme | Digestion pattern |
| EcoRI | 5' G|AATTC 3' 3' CTTAA|G 5' |
| HindIII | 5' A|AGCTT 3' 3' TTCGA|A 5' |
| BamHI | 5' G|GATCC 3' 3' CCTAG|G 5 |
| AluI | 5' AG|CT 3' 3' TC|GA 5' |
| SmaI | 5' CCC|GGG 3' 3' GGG|CCC 5' |
| HbaI | 5' GCG|C 3' 3' C|GCG 5' |
(The | symbol indicates the specific cut sites.)
Each answer may be used once, more than once, or not at all.
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