In your lab you have several mouse cell lines growing inculture, designated culture A through culture D. Some of these celllines are transformed and others are not. For each of thedescriptions below, tell whether the cell line is transformed ornormal, and explain your choice. If it is transformed, make aneducated guess about the gene involved in the transformation.
A. Cell line A is insensitive to the addition of growth factorsto the medium. If the cells are exposed to damaging radiation, thegrowth curve is unaffected. Many of the cells have abnormal numbersof chromosomes.
B. Cell line B will not grow suspended in liquid media. Thecells require a hard substrate, and then they only grow until onecell layer covers the substrate; they may grow a bit denser ifgrowth factors are added to the medium. The cells lose theirability to move once the substrate is covered with a monolayer ofcells.
C. Cell line C grows quickly and is quite successful when thecells are suspended in soft agar. The cells are impervious to theaddition of serum to their medium. Upon exposure to damagingradiation, most of the cells undergo apoptosis.
D. Cell line D grows quickly and is impervious to DNA damagingradiation, entering cycle after cycle of mitosis even after largedosis of radiation. If transfected with DNA containing a gene forp53, these cells will undergo apoptosis.
Help please. Will provide lots of points. The cell biology classuses the book Lodish Molecular Cell Biology 7th edition.
In your lab you have several mouse cell lines growing inculture, designated culture A through culture D. Some of these celllines are transformed and others are not. For each of thedescriptions below, tell whether the cell line is transformed ornormal, and explain your choice. If it is transformed, make aneducated guess about the gene involved in the transformation.
A. Cell line A is insensitive to the addition of growth factorsto the medium. If the cells are exposed to damaging radiation, thegrowth curve is unaffected. Many of the cells have abnormal numbersof chromosomes.
B. Cell line B will not grow suspended in liquid media. Thecells require a hard substrate, and then they only grow until onecell layer covers the substrate; they may grow a bit denser ifgrowth factors are added to the medium. The cells lose theirability to move once the substrate is covered with a monolayer ofcells.
C. Cell line C grows quickly and is quite successful when thecells are suspended in soft agar. The cells are impervious to theaddition of serum to their medium. Upon exposure to damagingradiation, most of the cells undergo apoptosis.
D. Cell line D grows quickly and is impervious to DNA damagingradiation, entering cycle after cycle of mitosis even after largedosis of radiation. If transfected with DNA containing a gene forp53, these cells will undergo apoptosis.
Help please. Will provide lots of points. The cell biology classuses the book Lodish Molecular Cell Biology 7th edition.
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