In your lab you have several mouse cell lines growing in culture, designated culture A through culture D. Some of these cell lines are transformed and others are not. For each of the descriptions below, tell whether the cell line is transformed or normal, and explain your choice. If it is transformed, make an educated guess about the gene involved in the transformation.
A. Cell line A is insensitive to the addition of growth factors to the medium. If the cells are exposed to damaging radiation, the growth curve is unaffected. Many of the cells have abnormal numbers of chromosomes.
B. Cell line C grows quickly. The cells are impervious to the addition of serum to their medium. Upon exposure to damaging radiation, most of the cells undergo apoptosis.
C. Cell line D grows quickly and is impervious to DNA damaging radiation, entering cycle after cycle of mitosis even after large dosis of radiation. If transfected with DNA containing a gene for p53, these cells will undergo apoptosis.
In your lab you have several mouse cell lines growing in culture, designated culture A through culture D. Some of these cell lines are transformed and others are not. For each of the descriptions below, tell whether the cell line is transformed or normal, and explain your choice. If it is transformed, make an educated guess about the gene involved in the transformation.
A. Cell line A is insensitive to the addition of growth factors to the medium. If the cells are exposed to damaging radiation, the growth curve is unaffected. Many of the cells have abnormal numbers of chromosomes.
B. Cell line C grows quickly. The cells are impervious to the addition of serum to their medium. Upon exposure to damaging radiation, most of the cells undergo apoptosis.
C. Cell line D grows quickly and is impervious to DNA damaging radiation, entering cycle after cycle of mitosis even after large dosis of radiation. If transfected with DNA containing a gene for p53, these cells will undergo apoptosis.
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Related questions
Question 10
A microscope image of a cell in interphase is treated with dyes to stain the nucleic acids (DNA and RNA). This image probably displays ______.
| visible nucleoli | ||
| separate visible chromosomes | ||
| visible spindle fibers |
1 points
Question 11
To observe plant cells undergoing mitosis, the best plant tissue to isolate would be ______.
| a section of the root tip | ||
| a section of the mature fruit | ||
| a section of the main stem |
1 points
Question 12
Owen observes a slide containing whitefish blastula sections. He finds a cell with visible spindle lines extending from two points on opposite sides of the cell and chromosomes as small black Xs halfway between the two poles. What stage of the cell cycle is Owen observing?
| anaphase | ||
| metaphase | ||
| prometaphase |
1 points
Question 13
What structural feature can you use to distinguish between DNA-stained onion cells in anaphase and telophase?
| Sister chromatids separate as anaphase ends and telophase begins. | ||
| The vesicles forming a cell plate appear around the onset of telophase. | ||
| The chromosomes are clustered at visible spindle poles during anaphase. |
1 points
Question 14
A carcinogen is any substance or environmental condition that ______.
| increases the frequency of mutation | ||
| causes direct damage to DNA | ||
| inhibits mitosis in cancer cells |
1 points
Question 15
Angelina observes a slide containing whitefish blastula sections. She finds a cell with visible spindle lines extending from two points on opposite sides of the cell and chromosomes as small black Xs halfway between the two poles. What intracellular structure forms each of the two points on opposite sides of this cell?
| a metaphase plate | ||
| a cell plate | ||
| a pair of centrioles |
1 points
Question 16
Which of the following best describes the relationship between interphase and the cell cycle?
| Interphase is a gap between rounds of cell division. | ||
| Interphase comprises a large majority of the cell cycle in almost all cells. | ||
| Interphase comprises a large majority of the cell cycle in slowly and non-dividing cells. |
Question 17
Radiation therapy and chemotherapy are cancer treatments that primarily attack cancer cells through ______.
| activating apoptosis in any rapidly dividing cells to kill off tumor cells | ||
| destroying the blood vessels in cancerous tumors to block the flow of nutrients | ||
| damaging DNA to the extent that cells die if they cannot repair the damage |
