(BIOCHEMISTRY QUESTION) Rotenone is a poison that acts by blocking electron flow in Complex I. Predict the effect of Rotenone on the following processes(for a)-d) you can simply state "up" or "down"): a) O2 utilization (up or down). b. ATP production(up or down). c. NADH levels (up or down). d, TCA cycle (inhibited or running). e. Finally, even after several hours of rotenone inhibition, will complex II continue to pass electrons from succinate to FAD (making FADH2) and finally ubiquinone? Why or why not?
MY RESPONSES -- could you review and let me know if they are correct? If not, could you provide the correct response with an explanation?
a. down
b. down
c. up, d. inhibited
e. no inhibition of complex leads to NADH accumulation, that leads to the inhibition of the citric acid cycle. If this cycle is inhibited then the production of succinate, the initial electron donor for complex II, will also be inhibited and thus complex II will no longer receive electrons in order to continue an electron transport.
(BIOCHEMISTRY QUESTION) Rotenone is a poison that acts by blocking electron flow in Complex I. Predict the effect of Rotenone on the following processes(for a)-d) you can simply state "up" or "down"): a) O2 utilization (up or down). b. ATP production(up or down). c. NADH levels (up or down). d, TCA cycle (inhibited or running). e. Finally, even after several hours of rotenone inhibition, will complex II continue to pass electrons from succinate to FAD (making FADH2) and finally ubiquinone? Why or why not?
MY RESPONSES -- could you review and let me know if they are correct? If not, could you provide the correct response with an explanation?
a. down
b. down
c. up, d. inhibited
e. no inhibition of complex leads to NADH accumulation, that leads to the inhibition of the citric acid cycle. If this cycle is inhibited then the production of succinate, the initial electron donor for complex II, will also be inhibited and thus complex II will no longer receive electrons in order to continue an electron transport.
