BIOS 220 Chapter Notes - Chapter 16: Base Pair, Dna Replication, Dna Mismatch Repair

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Chapter 16: 8, 13, 16, 23-24, 26, 31, 34. One would need to know the reading frame to figure out where to start reading the message. The mismatch repair happens by finding the delay in methylation of the new strand and then inserting the correct bases: bypass polymerases- replicate past damaged dna and stall replicative polymerases. They are different from replicative polymerases because they can have larger adducts on the bases. They are more error prone(no 3"-5" proofreading function) only add on few nucleotides before falling off. Mostly used to unblock replication fork and not to make long parts of dna: direct reversal, excision repair, transcription coupled repair, non homologous end joining, error free repair cant work since big eukaryotes do not replicate dna. This happens because there are no undamaged strands/sister chromatids available as templates for new dna synth: first there would be an a-> g change in the mrna explaining all 3 codon changes.

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