BIO SCI 97 Chapter Notes -Semiconservative Replication, Preimplantation Genetic Diagnosis, Neanderthal Genome Project
30 Jan 2014
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Document Summary
Pcr has revolutionized medicine, forensics, and experimental biology. Pre-implantation genetic diagnosis as an application of pcr. Screening blood products for diseases: viral infections in wild monkeys by collecting feces. 95 c denatures dna by breaking the hydrogen bonds between strands. 50-65 c temperature is reduced to allow primer to bind (anneal) to dna. 72 c temperature is raised to the optimum for the thermostable polymerase (taq) to extend the dna strand. Pcr polymerase chain reaction separate the two strands by heating it by 95c. Take the separated dna strands and add small dna primers that anneal to the complementary strands at a lower temperature so you get single stranded dna with short double stranded regions. 72c- taq polymerase extends the primer and makes a copy of the molecule. Trick of pcr you can repeat the process over and over again: requires a sequence-specific primer you have to know at least part of the dna sequence.
