BIO SCI 97 Lecture Notes - Lecture 6: Preimplantation Genetic Diagnosis, Kary Mullis, Nucleoside Triphosphate
2 Apr 2018
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Document Summary
Pcr: a revolutionary method developed by kary mullis in the 1980s. At the end of the pcr reaction, the speci c sequence will be accumulated in billions of copies (amplicons). Dna and there is enough dna to analyze and sequence the genome, which could be compared with the modern homosapien genomes- which ones we have inherited genetically) Start off with dna template, then you want to separate the two strands of dna in the test tube. Heat up the strands of dna to get rid of the hydrogen bonds. Pcr will amplify a particular region, and after heating up dna (denaturing it) you cool it down so nucleotides can base pair where they have complementary sequences (add. Polymerase/add heat- it will start at the 3 end of the template and extend 5 prime to 3 prime, incorporating new nucleotides). Polymerization always takes place 5 prime to 3 prime: the strand they are copying is going in the opposite direction.
