BIO374H5 Chapter Notes - Chapter 7: Monoclonal Antibody, Transcription Activator-Like Effector Nuclease, Rna Interference
Document Summary
Chapter 7: animal biotechnology: animals in research. In vitro testing not enough to determine impact of treatment on an entire organism. Model animal systems developed using both vertebrates (zebrafish) + invertebrates (fruit flies) + worms. Small possible to house large # in small spaces. Embryogenesis (earliy organ development) = ~ 120hrs. Gut, liver, + kidneys developed in first 48-72hrs. Lung+cardiovascular systems of dogs = similar to humans. Monkeys+chimpanzees for hiv/aids research b/c they are the only known animals that share humans" vulnerability to virus. Dogs,cats,primates make up <1% of total# of research animals. Monoclonal antibodies made by cell culture that involves fusing myeloma cells w/ mouse spleen cells immunized w/ desired antigen. Isolate monoclonals by a selective medium only fused cells can grow. Phase testing conduct statistically significant # of trials on cell cultures, live animals, and human research participants before products can be made available to the general public.
