Biochemistry 3381A Study Guide - Quiz Guide: Rna-Dependent Rna Polymerase, Viral Envelope, Membrane Fusion Protein
20 May 2020
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Roughly spherical (from em) when in cell culture: fresh clinical isolates: may be filamentous (morphology may vary) Envelope (unstable outside of host winter spread require close contact . Mediated m2 not involved: m2: proton channel allow rna release, acidify virion core > destabilise > uncoating (rna in cytoplasm, amantadine and rimantadine (flumadine): Inhibit m2 prevent uncoating: neuraminidase (na) exit. Beneath envelope: m1 (matrix protein, nucleoprotein (np) associated w/ genome, pb1, pb2 + pa: constitute viral rna dependent rna polymerase, packaged within virion convert -rna > mrna initiate replicative. 4 types: a-d: a = most human disease. Strain / isolates named by: type (a,b,c,d)/country of isolation / isolate no. Each segment = protein except: segment 7- spliced > m1 + m2, segment 8 ns1 + ns2. Mrna translated host ribosomes nuclear export necessary. Rna polymerase must separately produce precise +ve crna: used as template to make precise vrna copies, either packaged into new virions or templates > mrna.
