BIO 4115 Study Guide - Final Guide: Electrophoretic Mobility Shift Assay, C-Terminus, Rna Recognition Motif
29 Aug 2016
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7. lncrnas act as guides, decoys, enahncers, scaffolds. Northern blot: northern to detect specific rnas and info about length and abundance of gene x rna, oligomer probe. Rt-pcr products: create cdna, oligomer primers, whole population of rnas. Basis of regulation of rna level events: 1. In cis stem-loop structure base pairing within the rna strand. In trans - small reg rna encoded somewhere else base pairs element to be regulated: 2. Protein protein: possible that two proteins bind rna cis-elements and then bind each other bring 5/3" ends together. Ctd code and pre-mrna processing: ctd carboxy-terminal domain: 52 heptad repeats of ysptsps in humans, 26 in yeast, dynamic changes in it phosphorylation profile. Dynamic, synchronized process: mrna factory model cracking the ctd code. Activators (sr proteins) bind to enhancers (ese or ise) & repressors (hnrnps) bind to silencers (ess or iss) Generate suppressor mutations restoration of function starred: 5"-uguac-3" 3"-acaug-5", double mutant, splicing activity is restored, pseudorevertant .
