BIO 1140 Study Guide - Midterm Guide: Phosphatidylethanolamine, Primase, Membrane Potential

127 views4 pages

sapphiredonkey502

2 May 2015

School

University of Ottawa

Department

Biology

Course

BIO 1140

Professor

Caroline Petit- Turcotte

For unlimited access to Study Guides, a Grade+ subscription is required.

Document Summary

Previous midterm: distinguish between g-actin and a g protein, g-actin is a globular monomeric protein that when atp is bound to it polymerizes to form f-actin (filamentous actin) and microfilaments. G-protein is a heterotrimeric and activated when the g-protein coupled receptor that links to it is activated by its ligand. Individuals with xeroderma pigmentosum inherit a faulty dna repair mechanism. Bio1140: regulates gene transcription when activated by lipid-soluble messenger binding d, both b & d are true. Increase the length of the hc tails in its membrane phosphoglycerides. Increase the proportion of saturated hc tails in its membrane phosphoglycerides. Increase the amount of cholesterol in the membrane. Increase the proportion of phosphatidylcholine relative to phosphatidylethanolamine a. b. c. d: all of the above, adenylyl cyclase . Is a membrane-associated enzyme that produces the second messenger camp. Is an enzyme that catalyzes the transfer of a phosphate group from atp to a protein.

Related Questions

You decide to complete a summer undergraduate research project in a lab that studies G-Protein coupled receptor (GPCR) signaling. The laboratory you join has identified two new GPCRs, GPCR-A and GPCR-B. The same ligand binds each receptor, but two different heterotrimeric G-proteins are activated that regulate adenylyl cyclase. A. You find that activation of GPCR-A causes an increase in adenylyl cyclase activity, while activation of GPCR-B causes a decrease in adenylyl cyclase activity.

B. You have a cell line that expresses both GPCR-A, GPCR-B, the corresponding G proteins and adenylyl cyclase. There is a basal level of adenylyl cyclase activity that produces a baseline cAMP concentration. Your project is to characterize a series of mutations in these components. Will each mutation increase or decrease the intracellular levels of cAMP upon ligand addition (remember, both GPCR-A and GPCR-B bind the same ligand)?

a. A mutation in GPCR-A that prevents G-protein activation

b. A mutation in GPCR-B that prevents G protein activation

c. A mutation in Gsa that prevents release of bound GDP

d. A mutation in Gia that prevents GTP hydrolysis

EXPLAIN your thought process and why each one was answered as increase or decrease

erinlizard726

Cell biology Questions

1. Where is the sugar-phosphate backbone located in a DNA strand within the DNA double helix?

a) Alternates between outside and inside

b)Inside

c)Outside

d)Varies between purines and pyrimidines

e)Depends on the species

2) Which of these statements defines paracrine cell signaling?

A messenger molecule released into the blood stream to bind to a receptor on a cell far away from the release site.

A messenger molecule released by a cell into the extracellular space that binds to receptors on the surface of the same cell.

A messenger molecules released by a cell into the extracellular space that travels short distances to bind to a receptor in a nearby cell.

A messenger molecule release by a tissue into the lumen of an organ that diffuse to bind to receptors far away from the release site.

e)All of the above.

3. Which effect has the binding of the ligand to a G-protein coupled receptor on intracellular G-protein?

a)		ATP is hydrolyzed, activating phosphorylation
b)		GTP is exchanged for GDP on the G protein, inactivating the G protein
c)		GDP is exchanged for GTP on the G protein, activating the G protein
d)		GMP is released from the G-protein, inactivating the receptor
e)		None of the above

viridiangoat964

Question 22

What intrinsic enzymatic activity do G proteins have?

They are ATPases

They are glycosidases

They are GTP synthases

They are GTPases

Question 23

How are G proteins activated?

The GTP dissociates from the G protein and GDP associates with the G protein

The GDP dissociates from the G protein and GTP associates with the G protein

The G protein synthesizes GTP from GDP

The G protein synthesizes GTP from ATP

Question 24

How are G proteins inactivated?

Hydrolyze ATP

Bind GTP

Bind ATP

Hydrolyze GTP

Question 25

What is a GTPase activating protein (GAP)?

It hydrolyzes GTP

It promotes release of GDP and binding of GTP of a G protein

It speeds up the rate of GTPase activty of a G protein

Question 26

What is a guanine nucleotide exchange factor (GEF)?

It speeds up the rate of GTPase activty of a G protein

It hydrolyzes GTP

It promotes release of GDP and binding of GTP to a G protein

Question 27

Which of the following types of enzyme activate G proteins?

GAP

GEF

Question 28

Which of the following types of enzyme inactivate G-proteins?

GAP

GEF

Question 29

What is receptor internalization and recycling?

It is when the receptor is co-translationally imported into the ER before being degraded by the unfolded protein response

It is when the receptor is endocytosed and then the vesicle fuses with the endosome, releases ligand and the receptor is returned to the plasma membrane

It is when the receptor is digested with proteases into amino acids and those amino acids are used to synthesize a new receptor

It is when the receptor cycles in and out of the nucleus

Question 30

How do large proteins get into the nucleus?

Simple diffusion

Facilitated diffusion through the nuclear pore

Active transport through the nuclear pore

Question 31

How easy is it for the following reaction to occur?

ADP + Pi â ATP

This is very exergonic and highly favorable

This is very endergonic and highly unfavorable

This is slightly exergonic and somewhat favorable

Question 32

The hydrolysis of ATP is highly...

Exergonic

Endergonic

BIO 1140 Study Guide - Midterm Guide: Phosphatidylethanolamine, Primase, Membrane Potential

Document Summary

Get access

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Biology

Concepts of Biology

Essentials of Biology

Related Documents

BIO 1140 Quiz: Biolingo

BIO 1140 Lecture Notes - Lecture 6: Creb-Binding Protein, Adenylyl Cyclase, Signal Transduction

BIO 1140 Lecture Notes - Lecture 13: Protein Isoform, Blood Sugar, Apoptosis

Related Questions