From Table 1 Exp. 1, enter yes or no for each solution based on the presence of protein. Table 1: Testing for Proteins Results Sample Initial Color Final Color Protein Present (yes/no) 1 - Albumin Solution Question Blank 1 of 5 2 - Gelatin Solution Question Blank 2 of 5 3 - Glucose Question Blank 3 of 5 4 - Water Question Blank 4 of 5 5 - Unknown Question Blank 5 of 5
From Table 1 Exp. 1, enter yes or no for each solution based on the presence of protein. Table 1: Testing for Proteins Results Sample Initial Color Final Color Protein Present (yes/no) 1 - Albumin Solution Question Blank 1 of 5 2 - Gelatin Solution Question Blank 2 of 5 3 - Glucose Question Blank 3 of 5 4 - Water Question Blank 4 of 5 5 - Unknown Question Blank 5 of 5
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Experiment 1: Testing for Proteins
Data Tables
Table 1: Testing for Proteins Results
Sample | Initial Color | Final Color | Is Protein Present? |
---|---|---|---|
1. Albumin Solution | |||
2. Gelatin Solution | |||
3. Glucose | |||
4. Water | |||
5. Unknown |
Post-Lab Questions
Record your hypothesis about what will happen when Biuret solution is mixed with the solutions from test tubes 1, 2, 3, and 4 here. Be sure to use scientific reasoning to support your hypothesis.
Write a statement to explain the molecular composition of the unknown solution based on the results obtained during testing with the Biuret solution and each sample solution.
Diet and nutrition are closely linked to the study of biomolecules. How should you monitor your food intake to ensure the cells in your body have the materials necessary to function?
There are other types of reagents used to determine what type of biomolecule a substance is. For example, copper ions present in Benedictâs reagent reacts with the free end of any reducing sugars, such as glucose, when heated. Originally blue in color, these copper ions are reduced by the sugar, and produce an orange-red colored precipitate. Alternatively, iodine-potassium iodide (IKI) may also be used when working with starch. IKI contains special tri-iodine ions which interact with the coiled structure of a starch polymer. Prior to a reaction, the IKI displays a yellow-brown color; however, after reacting with starch, a dark purple or black color is presented.
The molecule pictured below produced a blue color when tested with Benedictâs reagent, a yellow color when tested with IKI, and a violet color when tested with Biuret reagent. Based on the structure shown below and these chemical results, what kind of biomolecule is this?
Table 4: Testing an Unknown - Proteins
Sample | Initial Color | Hypothesis (Protein +/-) | Final Color | Protein Present? |
Positive Control: | ||||
Negative Control: | ||||
Unknown |
Table 5: Testing an Unknown â ReducingSugars
- Describe the experimental protocol you developed and followedwhen testing the unknowns in each table. Be sure to include yourhypothesis.
Sample | Initial Color | Hypothesis (Reducing Sugar +/-) | Final Color | Reducing Sugar Present? |
Positive Control: | ||||
Negative Control: | ||||
Unknown |
Table 6: Testing an Unknown â Starch
Sample | Initial Color | Hypothesis | Final Color | Starch Present? |
Positive Control: | ||||
Negative Control: | ||||
Unknown |
Table 1: Testing for Proteins Results | |||
Sample | Initial Color | Final Color | Protein Present |
1 - Albumin Solution | |||
2 - Gelatin Solution | |||
3 - Glucose | |||
4 - Water | |||
5 - Unknown |
Post-Lab Questions
- Record your hypothesis about what will happen when Biuretsolution is mixed with the solutions from test tubes 1, 2, 3, and 4here. Be sure to use scientific reasoning to support yourhypothesis.
- Write a statement to explain the molecular composition of theunknown solution based on the results obtained during testing withthe Biuret solution and each sample solution.
- Diet and nutrition are closely linked to the study ofbiomolecules. How should you monitor your food intake to insure thecells in your body have the materials necessary to function?
- There are other types of reagents used to determine what typeof biomolecule a substance is. For example, copper ions present inBenedictâs reagent reacts with the free end of any reducing sugars,such as glucose, when heated. Originally blue in color, thesecopper ions are reduced by the sugar, and produce an orange-redcolored precipitate. Alternatively, iodine-potassium iodide (IKI)may also be used when working with starch. IKI contains specialtri-iodine ions which interact with the coiled structure of astarch polymer. Prior to a reaction, the IKI displays ayellow-brown color; however, after reacting with starch, a darkpurple or black color is presented.
The molecule pictured below produced ablue color when tested with Benedictâs reagent, a yellow color whentested with IKI, and a violet color when tested with Biuretreagent. Based on the structure shown below and these chemicalresults, what kind of biomolecule is this?