When a process is being automated it has to be optimized and validated. what is a gradient and how is it related to experiment design - optimization - validation of an assay being automated?

[A] USA
[B] China
[C] Israel
[D] UK

All of the following are positive signs regarding freedom to operate except:

a. The new invention is clearly superior than the prior art in terms of its utility, uniqueness, and non-obviousness

b. The existing patent has not been applied for or granted in the country where the company is seeking to operate

c. The existing patent has expired

d. The existing patent can be invalidated

[A] Cricket
[B] Food Processing
[C] COVID Vaccination
[D] Fuel Prices