Figure 2: Identify the tissue types below.
1-3: Identify the types of cartilage
4-6: Identify the types of muscle
7-11: Identify the types of connective tissue types below
12: Identify the tissue
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Hi, I really need up fiuring out how to do this. I'm doing a1:10 ratio dilution. I know it's regular chem. lab but I can'tremember. Equation and help please. Need by tonight.
Use 0.2 ml PCR tubes for these reactions. The thin wallfacilitates heat transfer. Be careful to not crush the tubesbetween your fingers. There is not much writing surface on thesetubes so it is best to create a log in your notebook and thennumber the tubes accordingly.
Reagent | Stock Conc | Final Conc | Dilution | Volume per Sample | Volume per Master Mix (N+1) |
Template DNA | 5 ul | ||||
5X GoTaq Flexi Buf | 5X | 1X | |||
dNTP Mixture | 10 mM | 0.2 mM | |||
MgCl2 | 25 mM | 2.5 mM | |||
D1S80 Fwd Primer | 10 uM | 0.5 uM | |||
D1S80 Reverse Primer | 10 uM | 0.5 uM | |||
GoTaq Hot Start Polymerase | 5U/ul | 1.25U | |||
MGW (Molecular Grade Water) |