1. Critically evaluate two different types of probes used in real time PCR with regard to various molecular research applications [40% of mark]
2. Explain the contribution of real-time PCR to the diagnosis of COVID-19 (SARS CoV-2 virus), compared to traditional PCR methods [60% of mark]

What is different about TCM observation diagnostic practice compared to traditional Chines Medicine?

Describe how the polymerase chain reaction works. Be sure tolist the various ingredients that are required to perform thepolymerase chain reaction. Explain the difference between end-pointand real- time (quantitative) PCR. Suggest an application foreach.