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Biology
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24 Dec 2019

The DNA fragments that are readable are separated via?

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Related questions

Restriction enzymes are used to construct restriction maps ofDNA. These are diagrams of specific DNA molecule that show thesites where the restriction enzymes cleave the DNA. To construct arestriction map, purified samples of the DNA are treated withrestriction enzymes, either alone or in combination, and then thereaction products are separated by agarose gel electrophoresis, atechnique that separates the DNA fragments based on size. (Thesmaller fragments travel more quickly and are found near the bottomof the gel, whereas the larger fragments are found near the top ofthe gel.)

Use the results of the agarose gel electrophoresis separation(above) to construct a restriction map for the sample of DNA.

Two "naked" (without histones or other proteins) double-stranded fragments of DNA are exactly the same length. At 89°C, fragment A has completely denatured, which means that the two strands have separated. At that temperature, fragment B is still double-stranded. How might these fragments differ, to result in different denaturation temperatures?

DNA Sequencing (Sanger method):

https://www.dnalc.org/resources/animations/sangerseq.html

Questions:

5. In what ways are PCR and Sanger sequencing similar?

6. What is the important difference in Sanger sequencing?

7. Why is size separation of DNA fragments so important in Sanger sequencing? How are the DNA fragments separated in the animation?

Additional videos on Sanger DNA sequencing:

Sanger Sequencing

https://www.youtube.com/watch?v=6ldtdWjDwes

The Sanger Method of DNA Sequencing

https://www.youtube.com/watch?v=FvHRio1yyhQ

____________________________________________________________________________

Bacterial Transformation:

https://www.dnalc.org/resources/animations/transformation1.html

Questions:

8. What are plasmids?

9. What was used to cut the plasmids?

10. What was used to seal the DNA fragments together?

11. How did the researchers select for bacteria that contain both antibiotic resistance genes?

12. What is a recombinant plasmid?

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