Question

1. Explain why topoisomerases areneeded in eucaryotic cells even though eucaryotic DNA molecules arelinear, not circular like procaryotic DNA molecules.

2.

a) What is the name of an antibacterial antibiotic which acts byaffecting topoisomerase?

b) What is the name of an anticancer drug which acts byaffecting topoisomerase?

3. Topoisomerases are involved in DNA replication, so itmakes sense that a drug which messes up topoisomerase activitystops the cell from reproducing. However, in many cases, the cellnot only stops reproducing but is also killed because its DNA isbroken. Suggest a mechanism for how messing up topoisomeraseactivity could cause this effect on the cell's DNA.