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What changes do the promoter and the polymerase undergo duringthe transition from a closed to an open complex. What drives thistransition in prokaryotes, and in eukaryotes? What is theimportance of the transition for the overall transcriptionprocess?

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Reid Wolff
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17 Dec 2019
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Related questions

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

1. How is the structure of eukaryotic DNA and chromosomes related to how transcription occurs?(Hint: histones and winding is important)

2.Compare RNA polymerase in bacteria to the RNA polymerase in eukaryotic cells? What are the structural differences? How does this relate to how each works?

3.Compare and contrast the promoters found in prokaryotes and eukaryotes.

4.How is initiation started in eukaryotes?

5.What are transcription factors and what role do they play?

6.How are elongation and termination similar in eukaryotes & prokaryotes? How do they differ?

Indicate whether eac is true for gene expression in prokaryotes,eukaryotes or both:

1. After transcription, a 3' poly- A tail and a 5' cap areaddeddto the RNA.

2. Translation of mRNA can begin before transcription iscomplete.

3. RNA polymerase binds to the promoter region to begintranscription

4. RNA polymerase can only add a robonucleotide to the 3' end ofthe growing RNA

5. The mRNA transcript is the exact complement of the gene fromwhich it was copied.

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