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12 Dec 2019

What is detected or determined with these methods?- (specific molecules location and use 5 words or fewer)
A. Confocal fluorescence microscopy

B. Northern blot and micro arrays

C. Western blot

D. Light microscope in phase contrast mode

E. southern blot

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Related questions

26. Which of the following is the best microscope to use to visualize a cross section of skin
stained so that the cells are purple and the extracellular matrix is pink:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
27. Which of the following is the best microscope to use in order to visualize cell to cell
junctions at high magnification:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
28. A student is studying the tensile strength of a new metallic alloy. He wants to visualize
the fracture surfaces following tensile testing. Which is the best microscope for this
application:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
29. A graduate student is studying the degradation properties of a new polymer that she
developed. She adds a fluorescent molecule to the polymer backbone and makes a thin
layer of the polymer on a glass slide. In order to visualize the polymer and measure its
degradation/disappearance over time, which is the best microscope to use:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
30. Endothelial cells were exposed to experimental conditions and are imaged intact under a
microscope following antibody labeling of NF-ÎºB, a protein located inside endothelial
cells. Select the following statement that best describes the approach that should be
used to image the endothelial cells in this experiment.
a. Cells are imaged under a transmission electron microscope.
b. Cells are imaged using phase-contrast in the light microscope.
c. Cells are imaged under a fluorescence microscope.
d. Cells are imaged under a scanning electron microscope.

26.The best microscopy technique for observing different subcellular structures on the surface of bacteria is

(A) SEM (B) TEM

(E) fluorescence microscopy

27.Which type of microscopy exploits differences in the refractive index of the specimen, where light is retarded by one-quarter of a wavelength when it passes through a bacterial cell?

(A) SEM (B) TEM

(E) fluorescence microscopy

28.Which type of microscopy should be used to examine bacterial cells that have been Gram stained?

(A) TEM (B) fluorescence microscopy

(E) bright-field microscopy

29.Which of the following is most abundant in a prokaryotic cell (in dry weight)?

(A) RNA (B) DNA

(E) Lipid

30.Which of the following techniques uses the Svedberg unit (S)?

(A) electron microscopy (B) ultracentrifugation

(E) sonication

31.Which of the following is NOT a function performed by the cell membrane or its components?

(A) production of proteins (B) detection of environmental signals

(E) B and D

32.Which of the following statement is true?

(A) Lipopolysaccharides (LPS) are found in the cell wall of Gram-negative bacteria.

(B) Lipopolysaccharides (LPS) are found in the cell wall of Gram-positive bacteria.

(D) Teichoic acids are found in the cell wall of Gram-positive bacteria.

(E) Cholesterol is found in the cell wall of Gram-negative bacteria.

33.Which of the following is found in Archaeabut not in Bacteria?

(A) Hopanoids (B) Terpenoids

(E) Peptidoglycan

34.The glycan chain of archaeal cell wall is made of __________ and __________, while the glycan chain of bacterial cell wall is made of __________ and __________.

(A) peptidoglycan and murein, pseudopeptidoglycan and murein

(B) N-acetylglucosamine and N-acetyltalosaminuronic acid,

N-acetylmuramic acid and N-acetyltalosaminuronic acid

N-acetylglucosamine and N-acetylmuramic acid

(D) N-acetylglucosamine and N-acetylmuramic acid,

N-acetylglucosamine and N-acetyltalosaminuronic acid

(E) N-acetylmuramic acid and N-acetyltalosaminuronic acid,

N-acetylglucosamine and N-acetyltalosaminuronic acid

35.The sacculus of bacteria is composed primarily of

(A) phospholipids (B) peptidoglycans

(E) nucleic acids

36.Lysozyme attacks the following linkage in the bacterial cell wall:

(A) Î²(1,4)-glycosidic bonds

(B) Î²(1,3)-glycosidic bonds

(D) Peptide cross-links between glycan chains

(E) Peptide bonds between L-alanine and D-glutamic acid

__________ are fairly small organelles that provide a safe place within the cell to carry out certain biochemical reactions that generate harmful, highly reactive oxygen species. These chemicals are both generated and broken down in the same location.

Lysosomes

Endosomes

Peroxisomes

Nucleosomes

Biologists cannot possibly study all living species. Instead, they try to understand cell behavior by studying a select subset of them. Which of the following characteristics are useful in an organism chosen for use as a model in laboratory studies?

rapid rate of reproduction

all of the above

ability to grow under controlled conditions

amenability to genetic manipulation

By definition, prokaryotic cells do not possess __________.

ribosomes.

a nucleus.

membrane bilayers.

replication machinery.

Cell biologists employ targeted fluorescent dyes or modified fluorescent proteins in both standard fluorescence microscopy and confocal microscopy to observe specific details in the cell. Even though fluorescence permits better visualization, the resolving power is essentially the same as that of a standard light microscope because the resolving power of a microscope is limited by the __________ of light.

intensity

absorption

wavelength

filtering

Choose the phrase that best completes this sentence: Microtubules ____________ and are required to pull duplicated chromosomes to opposite poles of dividing cells.

generate contractile forces

can rapidly reorganize

are found in especially large numbers in muscle cells

are intermediate in thickness

Despite the differences between eukaryotic and prokaryotic cells, prokaryotes have proteins that are distantly related to eukaryotic actin filaments and microtubules. What is likely to be the most ancient function of the cytoskeleton?

cell division

cell motility

vesicle transport

membrane support

DNA and RNA are different types of nucleic acid polymer. Which of the following is true of DNA but not true of RNA?

It contains thymine.

It has 5'-to-3' directionality.

It is single-stranded.

It contains uracil.

Double covalent bonds are both shorter and stronger than single covalent bonds, but they also limit the geometry of the molecule because they ____________________.

prevent additional bonds from being formed with the bonded atoms.

limit the rotation of the bonded atoms.

create a new arrangement of electron shells.

change the reactivity of the bonded atoms.

Drosophila melanogaster is a/an __________. This type of animal is the most abundant of all animal species, making it an appropriate choice as an experimental model.

mammal

amphibian

bird

insect

10.

If the isotope 32S has 16 protons and 16 neutrons, how many protons, neutrons, and electrons will the isotope 35S have, respectively?

16, 19, 17

16, 19, 15

16, 19, 16

16, 20, 15

11.

Living systems are incredibly diverse in size, shape, environment, and behavior. It is estimated that there are between 10 million and 100 million different species. Despite this wide variety of organisms, it remains difficult to define what it means to say something is alive. Which of the following can be described as the smallest living unit?

cell

organelle

protein

DNA

12.

Macromolecules in the cell can often interact transiently as a result of noncovalent interactions. These weak interactions also produce stable, highly specific interactions between molecules. Which of the factors below is the most significant in determining whether the interaction will be transient or stable?

the concentration of each molecule

the size of each molecule

surface complementarity between molecules

the rate of synthesis

13.

Many of the mechanisms that cells use for maintenance and reproduction were first studied at the molecular level in bacteria. Which bacterial species had a central role in advancing the field of molecular biology?

E. coli

S. pombe

C. elegans

D. melanogaster

14.

Many types of cells have stores of lipids in their cytoplasm, usually seen as fat droplets. What is the lipid most commonly found in these droplets?

cholesterol

isoprene

triacylglycerol

palmitic acid

15.

Mitochondria contain their own genome, are able to duplicate, and actually divide on a different time line from the rest of the cell. Nevertheless, mitochondria cannot function for long when isolated from the cell because they are __________________.

anaerobes.

viruses.

parasites.

endosymbionts.

16.

Mitochondria perform cellular respiration, a process that uses oxygen, generates carbon dioxide, and produces chemical energy for the cell. Which answer below indicates a correct pairing of material âburnedâ and the form of energy produced during cellular respiration?

17.

Polar covalent bonds are formed when the electrons in the bond are not shared equally between the two nuclei. Which one of these molecules contains polar bonds?