Question

I need help with the following question. Not so much with the first portion, but part B. Please fully explain how you derived each value used to determine the ultimate Delta G. Use 298K as the temperature. I'm really lost so thank you in advance!

Lactic acid fermenation.

(a) Write a balanced equation for the conversion of glucose into lactate.

-( Glucose +2Pi + 2 ADP ---> 2 Lactate +2ATP +2H2O)

(b) Calculate the standard free energy change of this reaction by using the following data the fact that Delta G naught prime is -25kj/mol for the following reaction:

Pyruvate + NADH + H+<---> lactate +NAD+

What is the free energy change (Delta G not Delta G naught prime) of this reaction when the concentrations of reactant are glucose= 5mM, lactate=0.05, ATP=2mM, ADP=0.2mM, and Pi=1mM?

Date given from table:

Reaction 1 Glucose ATP glucose 6-phosphate AD t H Glucose 6-phosphate fructose 6-phosphate 3 Fructose 6-phosphate t ATP- fructose 1,6-bisphosphate t AD 4 Fructose 1,6 bisphosphate dihydroxy acetone phosphate glycerald 5 Dihydroxyacetone phosphate 3.phosph 6 Glyceraldehyde 3-phosphate tr Pi t NAD 1,3 bisphosphoglycerate 1.3 phosphoglycerate ADP phosphoglycerate 8 phosphoglycerate H 2-Phosphoglycerate phosphoenolpyruvate 10 Phosphoenolpyruvate t ADP t H pyruvate ATP Note: AG the actual free change, has been calculated from AG and k reactants under typical physiological conditions, Glycolysis can proceed only that the of are negative. The small positive values of three of the above reactions indicate of metabolites in vivo in cells undergoing glycolysis are not precisely known. AG" in kj mol AG in mol (kcal mol (kcal mol 1) Reaction type Enzyme Phosphoryl transfer 6.7 33.5 8.0 0.4) 2.5 (-0,0) Isomerization Phosphoryl transfer 22.2 (-5.3 phosphofructokinase 1.30-0.3) +23.8 (+5.7) Aldol cleavage Triose phosphate isomerase Isomerization coupled phosphate 'oxidation rate kinase Phosphoryl transfer Phosphoglycerate mutase Phosphoryl shift Dehydration +7.5 (+1.8) 2.5 t 0.6 1.7 (-0.4) +6.3 (+1.5) 18.8 (-4.5) t 1.3 (+0.3) t4.6 (+1.1) +0.8 (+0.2) +1.7 (+0.4) 3.30-0.8) -16 (-4.0) 31.4 (-7.5) Phosphoryl transfer kinase Pyruvate NADH, in a reaction catalyzed by alcohol dehydrogenase. This reaction by

Show transcribed image text Reaction 1 Glucose ATP glucose 6-phosphate AD t H Glucose 6-phosphate fructose 6-phosphate 3 Fructose 6-phosphate t ATP- fructose 1,6-bisphosphate t AD 4 Fructose 1,6 bisphosphate dihydroxy acetone phosphate glycerald 5 Dihydroxyacetone phosphate 3.phosph 6 Glyceraldehyde 3-phosphate tr Pi t NAD 1,3 bisphosphoglycerate 1.3 phosphoglycerate ADP phosphoglycerate 8 phosphoglycerate H 2-Phosphoglycerate phosphoenolpyruvate 10 Phosphoenolpyruvate t ADP t H pyruvate ATP Note: AG the actual free change, has been calculated from AG and k reactants under typical physiological conditions, Glycolysis can proceed only that the of are negative. The small positive values of three of the above reactions indicate of metabolites in vivo in cells undergoing glycolysis are not precisely known. AG" in kj mol AG in mol (kcal mol (kcal mol 1) Reaction type Enzyme Phosphoryl transfer 6.7 33.5 8.0 0.4) 2.5 (-0,0) Isomerization Phosphoryl transfer 22.2 (-5.3 phosphofructokinase 1.30-0.3) +23.8 (+5.7) Aldol cleavage Triose phosphate isomerase Isomerization coupled phosphate 'oxidation rate kinase Phosphoryl transfer Phosphoglycerate mutase Phosphoryl shift Dehydration +7.5 (+1.8) 2.5 t 0.6 1.7 (-0.4) +6.3 (+1.5) 18.8 (-4.5) t 1.3 (+0.3) t4.6 (+1.1) +0.8 (+0.2) +1.7 (+0.4) 3.30-0.8) -16 (-4.0) 31.4 (-7.5) Phosphoryl transfer kinase Pyruvate NADH, in a reaction catalyzed by alcohol dehydrogenase. This reaction by