For an Î±-helical polyalanine peptide 45.00 residues in length, calculate the melting temperature in Â°C. Tm= Â°C

Calculate the melting temperature of a 45.00 amino acid Î±-helical peptide if that peptide contains all alanines and a single internal glycine residue. Tm= Â°C

Calcuate the melting temperature if the 45.00 amino acid Î±-helical peptide contains 5.00 internal glycine residues and a glycine at the N-terminus of the helix. Tm= Â°C

Calculate the melting temperature for a 45.00 amino acid polyhistidine peptide at physiological pH in a 310-helix. Tm= Â°C

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Casey DurganLv2

27 May 2019

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6. (10 points) When double-stranded DNA is heated, the two strands separate into single strands in a process called melting or denaturation. The temperature at which half of the duplex DNA molecules are intact and half have melted is defined as the Tm.

a. Do you think Tm is a constant, or can it depend on other small molecules in the solution? Do you think high salt concentrations increase, decrease, or have no effect on Tm

b. Under standard conditions, the expected melting temperature in degrees Celsius can be calculated from the equation Tm = 59.9 + [0.41 Ã %(G + C)] â (675/length of duplex). Does the Tm increase or decrease if there are more G + C (and thus fewer A + T) base pairs? Does the Tm increase or decrease as the length of DNA increases? Why?

c. Calculate the predicted Tm for a stretch of double helix that is 100 nucleotides long and contains 50% G + C content.

When double-stranded DNA is heated, the two strands separate into single strands in a process called melting or denaturation. The temperature at which half of the duplex DNA molecules are intact and half have melted is defined as the T_m.

A. Do you think T_m is a constant, or can it depend on other small molecules in the solution? Do you think high salt concentrations increase, decrease, or have no effect on T_m?

B. Under standard conditions, the expected melting temperature in degrees Celsius can be calculated from the equation T_m = 59.9 + [0.41 Ã %(G + C)] â (675/length of duplex). Does the T_m increase or decrease if there are more G + C (and thus fewer A + T) base pairs? Does the T_m increase or decrease as the length of DNA increases? Why?

C. Calculate the predicted T_m for a stretch of double helix that is 100 nucleotides long and contains 50% G + C content.

1. The level of protein structure that is stabilized primarilyby non-covalent interactions between the side chains within asingle polypeptide chain is refered to as its:

A) primary structure

B) secondary structure

C) tertiary structure

D) quaternary structure

E) none of the above

2. Collagen, a strong fibrous protein found in connectivetissue, bone, and the extracellular matrix, has a structure inwhich three left-handed helices wind around each other to form aright-handed triple helix. The primary structure of collagen has arepeat unit of -(Gly-X-Pro)- Which of the following bonds stabilizethe collagen triple helix? (mark all that apply)

A) Hydrogen bonds between hydroxyproline residues

B) Hydrogen bonds between N-H and C=O

C) Ionic bonds between amino acid side chains

D) Hydrogen bonds between charged amino acids

3. The non-covalent forces that stabilize the Beta-strandconformation of peptides include:

A) H-bonds between amide C=O and amide NH

B) Van der Waals packing between the peptide backbone atoms

C) Van der Waals packing due to side chain interdigitation

D) Only A and B above

E) None of the above

4. What is the approximate length difference of a 22-kDasingle-stranded ?-helical protein segment vs. a 22-kDasingle-stranded ?-strand protein segment? Assume a mean residuemass of 110 Da. Show work.

5. Circular dichroism measurements have shown that the peptidebackbone of poly-l-lysine (KKKKKKK) adopts a random coilconformation at pH 7.0, but becomes ?-helical as the pH is raisedabove 10. This observation is known as the