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8 Dec 2018

DNA Recombination

5. List some of the proteins that are involved in homologous recombination (see Table 20.1).

6. Which repair mechanisms are involved in gene conversion? What exactly is “gene conversion” anyway?

7. What is the example given for site-specific recombination?

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Sixta Kovacek
Sixta KovacekLv2
11 Dec 2018

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Related questions

12. During homologous recombination, what events are required for formation of the heteroduplex?

13. Assume that homologous recombination occurs across a region of DNA containing a wild-type and mutant allele of a particular gene. There is a single base pair change in the mutant allele as follows:

wild-type = 5’…TTGGGTA… à 5’…TTGCGTA… = mutant allele, such that the resulting dsDNA is as follows:

5’…TTGGGTA…

3’…AACGCAT…

What would be the genotype (wild-type or mutant) if base excision repair occurs on the TOP strand?

14. What term describes the process occurring above?

1) An individual is heterozygous for 3 genes (A/a B/b and C/c). He is crossed to an individual that is homozygous recessive for the same 3 genes (a/a b/b and c/c). The following offspring were seen. Which of the following phenotypes represent a double crossover event? (Hint - are you seeing all of the phenotypes in the next generation that you expected to see?) Select all that apply.

Phenotype Number

+++

154

+b+

322

a+c

318

abc

148

++c

76

ab+

82

2) Use the same information in the question above. What is the gene order?

The gene order is B-C-A

The gene order is A-B-C

The gene order is B-A-C

3) Using the same information as in the previous problem, What is the recombination frequency between each gene pair? Round your answer to 2 decimal places. For example, report 12.2345% as 0.12.

recombination frequency between A/B =

recombination frequency between A/C =

recombination frequency between B/C =

******According to the hint, we don't see two phenotypes that we are expecting which are +bc, and a++. Are those the ones that represent double crossovers, and if so, how do we calculate the recombination frequency between each gene? Would very much appreciate some help.

Part A- Microtubules. Select all that apply.

1) They are filaments in mitotic and meiotic spindles

2) They are required for moving sister chromatids to opposite sides of the poles during anaphase II of meiosis and anaphase of mitosis.

3) They are required for moving homologs to opposite sides of the poles during anaphase I of meiosis.

4) They are important for maintaining homologous chromosome pairing and plays a role in recombination.

5) They catalyze the phosphorylation of specific proteins.

6) They are a layered structue located between paired homologs in prophase I of meiosis in many organisms.

Part B- Cyclin-dependent kinases. Select all that apply.

1) They are filaments in mitotic and meiotic spindles.

2) They are enzymes that control progression through cell cycle.

3) They are required for the segregation of chromosomes during anaphase of mitosis or meiosis.

4) They are important for homologous chromosome pairing and plays a role in recombination.

5) They catalyze the phosphorylation of specific proteins.

Part C- Kinetochores. Select all that apply.

1) They are enzymes that control progression through cell cycle.

2) They are important for maintaining homologous chromosome pairing and plays a role in recombination.

3) They are required for the segregation of chromosomes during anaphase of mitosis or meiosis.

4) They are complx structures attached to the centromeres of chromosomes that bind to spindle microtubules.

5) They catalyze the phosphorylation of specific proteins.

Part D- Synaptonemal complex. Select all that apply.

1) It is required for moving sister chromatids to opposite sides of the poles during anaphase II of meiosis and anaphase of mitosis.

2) It is a layered structure located between paired homologs in prophase I of meiosis in many organisms.

3) It is complex structures attached to the centromeres of chromosomes that bind to spindle microtubules.

4) It catalyzes the phospohorylation of specific proteins.

5) It is important for maintaining homologous chromosome pairing and plays a role in recombination.

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