can someone explain HOW GAPDH 'normalizes' mRNA levels of genes of interest in a RT-qPCR (like what does normalize mean)

answer

watching

views

For unlimited access to Homework Help, a Homework+ subscription is required.

You have 0 free answers left.
Get unlimited access to 3.8 million step-by-step answers.

Get unlimited access

Already have an account? Log in

Bunny GreenfelderLv2

27 Mar 2019

Unlock all answers

Get 1 free homework help answer.

Already have an account? Log in

Related questions

if the purpose of a RT-qPCR is to normalize mRNA in order to have a proper comparison/quantification of mRNA levels, why is the ending result DNA or more so, how does that tie into the normalized mRNA levels that are being expressed?

What should the Ct values of a negative control be from an RT-qPCR? I got values ranging from 30.0-35.0, while in example problems I've noticed they are usually labeled "undetermined," does this mean my results show I've done my experiment incorrectly?

. What is an mRNA?

2. How does the RT-PCR tell you if a gene is expressed in a particular cell type?

3. Where can RNases be found? How can you prevent RNase contamination in your samples during experiments involving RNA?

raspberrycamel466

Weekly leaderboard

Home

Homework Help 3,900,000

Biology 460,000

can someone explain HOW GAPDH 'normalizes' mRNA levels of genes of interest in a RT-qPCR (like what does normalize mean)

Unlock all answers

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Biology

Concepts of Biology

Essentials of Biology

Related questions

Related Documents

BIO 1140 Chapter Notes -Golgi Apparatus, Lac Operon, Microbody

BIO373H5 Chapter Notes - Chapter 6.6: Fluorescent Tag, Shotgun Sequencing, Temperature Gradient Gel Electrophoresis

BIO 319 Lecture Notes - Lecture 3: Cloning Vector, Plasmid, Real-Time Polymerase Chain Reaction

Weekly leaderboard