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24 Jan 2018

Briefly define what is meant by negative and positive control of the lac operon.

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Keith Leannon
Keith LeannonLv2
25 Jan 2018
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Related questions

The lac operon is under both negative( the repressor) and positive ( CPR) control. Why do you think this is? Why would this be beneficial?

When glucose is present, the lac genes are not fully expressed, even when lactose is present to repress the Lac repressor.
(a) Why does it make biological sense to have the lac operon be sensitive to the presence of glucose? How is this accomplished?
(b) Why does it make biological sense to have the lac operon be under the negative control of the Lac repressor?

5.) Is the CAP/cAMP effect on the transcription of the lac operon an example of positive or negative regulation? Why?

6.) Explain what happens during catabolite repression of the lac operon when both Glucose and Lactose are in high amounts. What is the biological relevance of this control?

7.) Why are Oc mutations said to be “dominant” over Is mutants?

8.) The rate of transcription of the trp operon in E. coli is controlled by what two regulatory processes? These two processes can regulate the trp operon up to about _____-fold due the combined effects of these processes. By what mechanisms do these two regulatory processes modulate trp operon transcript levels?

9.) Explain how high levels of tryptophan in the environment in which an E. coli cell is growing results in premature termination of transcription of the trp operon.

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